About: Sample drift correction in 3D fluorescence photoactivation localization microscopy

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About: Sample drift correction in 3D fluorescence photoactivation localization microscopy Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The recent development of diffraction-unlimited far-field fluorescence microscopy has overcome the classical resolution limit of similar to 250 nm of conventional light microscopy by about a factor of ten. The improved resolution, however, is susceptible to sample drift on a much finer scale than previously relevant. Here we discuss an approach that corrects for three-dimensional (3D) drift in images of fixed samples without the requirement for fiduciary markers or instrument modification. By imaging mitochondria with Biplane FPALM we show our algorithm’s feasibility in a practical application The recent development of diffraction-unlimited far-field fluorescence microscopy has overcome the classical resolution limit of similar to 250 nm of conventional light microscopy by about a factor of ten. The improved resolution, however, is susceptible to sample drift on a much finer scale than previously relevant. Here we discuss an approach that corrects for three-dimensional (3D) drift in images of fixed samples without the requirement for fiduciary markers or instrument modification. By imaging mitochondria with Biplane FPALM we show our algorithm’s feasibility in a practical application (en)
Title	Sample drift correction in 3D fluorescence photoactivation localization microscopy Sample drift correction in 3D fluorescence photoactivation localization microscopy (en)
skos:prefLabel	Sample drift correction in 3D fluorescence photoactivation localization microscopy Sample drift correction in 3D fluorescence photoactivation localization microscopy (en)
skos:notation	RIV/67985823:_____/11:00370168!RIV12-AV0-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP302/10/0346), P(KJB500110902), P(ME09029), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika	16
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Ježek, Petr Šantorová, Jitka Smolková, Katarína Dlasková, Andrea
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	228121
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/11:00370168
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Biplane FPALM microscopy; mitochondria; sample drift correction (en)
http://linked.open.../riv/klicoveSlovo	Biplane FPALM microscopy sample drift correction mitochondria
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[878B84D16F3E]
http://linked.open...i/riv/nazevZdroje	Optics Express
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	3D imaging of mitochondrial network and ultrastructure in relation to bioenergetics, hypoxia, and diseases Mitochondrial DNA manipulations for simulation of oxidative stress Imaging of mitochondrial substructure by 3D high resolution microscopy
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Ježek, Petr Bewersdorf, J. Callahan, S. P. Dlasková, Andrea Mlodzianoski, M. J. Schreiner, J. M. Smolková, Katarína Šantorová, Jitka
http://linked.open...ain/vavai/riv/wos	000293339200025
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka
issn	1094-4087
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1364/OE.19.015009

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