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Description
  • In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain
  • In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain (en)
Title
  • Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
  • Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy (en)
skos:prefLabel
  • Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
  • Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy (en)
skos:notation
  • RIV/67985823:_____/11:00369489!RIV12-AV0-67985823
http://linked.open...avai/riv/aktivita
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  • P(GA102/08/0691), P(GA304/09/0733), P(LC06063), P(ME09010), Z(AV0Z50110509), Z(MSM6840770012)
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
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  • 191331
http://linked.open...ai/riv/idVysledku
  • RIV/67985823:_____/11:00369489
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • confocal laser scanning microscopy; image enhancement; morphology filters (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [EE008501A8A4]
http://linked.open...i/riv/nazevZdroje
  • Microscopy Research Technique
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http://linked.open...v/svazekPeriodika
  • 74
http://linked.open...iv/tvurceVysledku
  • Kubínová, Lucie
  • Michálek, Jan
  • Čapek, Martin
http://linked.open...ain/vavai/riv/wos
  • 000294401200007
http://linked.open...n/vavai/riv/zamer
issn
  • 1059-910X
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/jemt.20965
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