About: The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod

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About: The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We compared two methods of evaluation of capillary supply of individual skeletal muscle fibers, the traditional one using 2D images of thin transverse sections by the number of capillary profiles around a fiber (CAF) and the 3D approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed We compared two methods of evaluation of capillary supply of individual skeletal muscle fibers, the traditional one using 2D images of thin transverse sections by the number of capillary profiles around a fiber (CAF) and the 3D approach where the mean length of capillaries around individual muscle fibers per fiber length (Lcap/Lfib) is measured from 3D images acquired by confocal microscopy. In models we showed that Lcap/Lfib was sensitive to different arrangements of capillaries, while CAF underestimated capillarization since it could not detect the increased length of capillary bed. In true muscle samples, we detected statistically significant differences in the capillary supply of control and denervated rat soleus muscles by both 2D and 3D methods. Lcap/Lfib was larger than CAF in control muscles reflecting their more complicated capillary bed (en)
Title	The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod (en)
skos:prefLabel	The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod The estimation error of skeletal muscle capillary supply is significantly reduced by 3D Metod (en)
skos:notation	RIV/67985823:_____/10:00341849!RIV11-GA0-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD204/09/H084), P(LC06063), P(MEB090910), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Janáček, Jiří Kubínová, Lucie Vyhnal, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	257544
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/10:00341849
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	confocal microscopy; capillaries; length measurement (en)
http://linked.open.../riv/klicoveSlovo	length measurement capillaries confocal microscopy
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[B4FC84DA7392]
http://linked.open...i/riv/nazevZdroje	Microvascular Research
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Molecular biology of a differentiating cell The role of distal nerve stump on remodelling of capillary network and myosin heavy chain isoform transformation in denervated rat skeletal muscle.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Eržen, I. Janáček, Jiří Kubínová, Lucie Vyhnal, Aleš Čebašek, V. Ribarič, S.
http://linked.open...ain/vavai/riv/wos	274055200006
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka
issn	0026-2862
number of pages	7 (xsd:int)

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