About: Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations

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About: Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	S. cerevisiae mutant strains lacking the genes encoding alkali-metal-cation exporters (NHA1, ENA1, ENA2, ENA5, TOK1) and potassium importers (TRK1, TRK2) have been constructed. The TRK1 gene has been substituted by the LEU2 gene; the three ENA genes, TOK1 and TRK2 genes have been replaced by the kanMX cassette. Finally, the LEU2 and kanMX cassettes from all strains were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background lacking either 6 or 7 transporters have been created to study heterologously expressed sodium and potassium transporters from both lower and higher eukaryots S. cerevisiae mutant strains lacking the genes encoding alkali-metal-cation exporters (NHA1, ENA1, ENA2, ENA5, TOK1) and potassium importers (TRK1, TRK2) have been constructed. The TRK1 gene has been substituted by the LEU2 gene; the three ENA genes, TOK1 and TRK2 genes have been replaced by the kanMX cassette. Finally, the LEU2 and kanMX cassettes from all strains were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background lacking either 6 or 7 transporters have been created to study heterologously expressed sodium and potassium transporters from both lower and higher eukaryots (en)
Title	Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations (en)
skos:prefLabel	Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations (en)
skos:notation	RIV/67985823:_____/09:00337845!RIV10-MSM-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/08/0354), P(IAA500110801), P(LC531), Z(AV0Z50110509)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Herynková, Pavla Petrezsélyová, Silvia Sychrová, Hana
http://linked.open.../riv/druhVysledku	G/B - Funkční vzorek
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...onomickeParametry	náklady v 300 000,- Kč, licenční poplatek 50 000,- Kč
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	316175
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/09:00337845
http://linked.open...terniIdentifikace	S. cerevisiae BYT1245 a BYT12345
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open...vai/riv/kategorie	A - Do 5 mil. Kč
http://linked.open.../riv/klicovaSlova	yeast heterologous expression; S. cerevisiae; salt-sensitive mutant (en)
http://linked.open.../riv/klicoveSlovo	S. cerevisiae yeast heterologous expression salt-sensitive mutant
http://linked.open...ontrolniKodProRIV	[918532ABC140]
http://linked.open.../licencniPoplatek	N - Poskytovatel licence na výsledek nepožaduje licenční poplatek
http://linked.open...okalizaceVysledku	Fyziologický ústav AVČR, v.v
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Alkali-metal-cation homeostasis in Saccharomyces cerevisiae biology Centre on molecular biology and physiology of yeast communities Role of Na/H antiporters in cell physiology - transporters teamwork in intracellular pH and K+ homeostasis
http://linked.open...UplatneniVysledku	2009
http://linked.open...echnickeParametry	Mutantní kmeny S. cerevisiae BYT1245 (nha1 ena1-5 trk1 trk2) a BYT12345 (nha1 ena1-5 tok1 trk1 trk2) s vysokou citlivostí k solím alkalických kationů a zároveň s neschopností růst na médiích s limitně nízkým obsahem draselných solí
http://linked.open...iv/tvurceVysledku	Petrezsélyová, Silvia Sychrová, Hana Herynková, Pavla
http://linked.open...avai/riv/vlastnik	Fyziologický ústav AVČR, v.v
http://linked.open...itiJinymSubjektem	P - Nabytí licence je nutné v některých případech
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka

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