About: Genetically modified S. cerevisiae strain lacking plasma membrane uptake systems for potassium cations

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	S.cerevisiae BYT1, BYT2 and BYT12 mutant strains lacking the TRK1 and/or TRK2 genes (encoding potassium uptake transporters) have been constructed. The TRK1 gene has been substituted by the LEU2 gene and the TRK2 has been substituted by the kanMX cassette. Finally, LEU2 and kanMX cassettes were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background have been created to study heterologously expressed potassium transporters from both lower and higher eukaryotes S.cerevisiae BYT1, BYT2 and BYT12 mutant strains lacking the TRK1 and/or TRK2 genes (encoding potassium uptake transporters) have been constructed. The TRK1 gene has been substituted by the LEU2 gene and the TRK2 has been substituted by the kanMX cassette. Finally, LEU2 and kanMX cassettes were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background have been created to study heterologously expressed potassium transporters from both lower and higher eukaryotes (en)
Title	Genetically modified S. cerevisiae strain lacking plasma membrane uptake systems for potassium cations Genetically modified S. cerevisiae strain lacking plasma membrane uptake systems for potassium cations (en)
skos:prefLabel	Genetically modified S. cerevisiae strain lacking plasma membrane uptake systems for potassium cations Genetically modified S. cerevisiae strain lacking plasma membrane uptake systems for potassium cations (en)
skos:notation	RIV/67985823:_____/09:00337841!RIV10-MSM-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/08/0354), P(LC531), Z(AV0Z50110509)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Herynková, Pavla Petrezsélyová, Silvia Sychrová, Hana
http://linked.open.../riv/druhVysledku	G/B - Funkční vzorek
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...onomickeParametry	náklady v 300 000,- Kč, licenční poplatek 50 000,- Kč
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	316176
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/09:00337841
http://linked.open...terniIdentifikace	S. cerevisiae BYT1, BYT2 a BYT12
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open...vai/riv/kategorie	A - Do 5 mil. Kč
http://linked.open.../riv/klicovaSlova	yeast heterologous expression; S. cerevisiae; plasma-membrane potassium transport system (en)
http://linked.open.../riv/klicoveSlovo	plasma-membrane potassium transport system S. cerevisiae yeast heterologous expression
http://linked.open...ontrolniKodProRIV	[9B7D6E31B8D9]
http://linked.open.../licencniPoplatek	N - Poskytovatel licence na výsledek nepožaduje licenční poplatek
http://linked.open...okalizaceVysledku	Fyziologický ústav AVČR, v.v
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Alkali-metal-cation homeostasis in Saccharomyces cerevisiae biology Centre on molecular biology and physiology of yeast communities
http://linked.open...UplatneniVysledku	2009
http://linked.open...echnickeParametry	Mutantní kmeny S. cerevisiae BYT1, BYT2 a BYT12 s neschopností růstu na médiích s limitně nízkým obsahem draselných solí a hyperpolarizovanou plasmatickou membránou jsou vhodné pro heterologní expresi transportérů draslíku z jiných eukaryot
http://linked.open...iv/tvurceVysledku	Petrezsélyová, Silvia Sychrová, Hana Herynková, Pavla
http://linked.open...avai/riv/vlastnik	Fyziologický ústav AVČR, v.v
http://linked.open...itiJinymSubjektem	P - Nabytí licence je nutné v některých případech
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka

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