About: Genetically modified S. cerevisiae BY471-derived strains lacking plasma membrane efflux systems for potassium cations

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About: Genetically modified S. cerevisiae BY471-derived strains lacking plasma membrane efflux systems for potassium cations Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	S. cerevisiae BYT4, BYT5 and BYT45 mutant strains lacking the alkali-metal-cation exporters encoded by ENA (ENA1, ENA2, ENA5) and NHA1 genes have been constructed. All genes have been substituted by the kanMX cassette. Finally, kanMX cassettes from all strains were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background have been created to study heterologously expressed sodium and potassium transporters from both lower and higher eukaryotes S. cerevisiae BYT4, BYT5 and BYT45 mutant strains lacking the alkali-metal-cation exporters encoded by ENA (ENA1, ENA2, ENA5) and NHA1 genes have been constructed. All genes have been substituted by the kanMX cassette. Finally, kanMX cassettes from all strains were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background have been created to study heterologously expressed sodium and potassium transporters from both lower and higher eukaryotes (en)
Title	Genetically modified S. cerevisiae BY471-derived strains lacking plasma membrane efflux systems for potassium cations Genetically modified S. cerevisiae BY471-derived strains lacking plasma membrane efflux systems for potassium cations (en)
skos:prefLabel	Genetically modified S. cerevisiae BY471-derived strains lacking plasma membrane efflux systems for potassium cations Genetically modified S. cerevisiae BY471-derived strains lacking plasma membrane efflux systems for potassium cations (en)
skos:notation	RIV/67985823:_____/09:00337839!RIV10-MSM-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/08/0354), P(LC531), Z(AV0Z50110509)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Herynková, Pavla Sychrová, Hana Zahrádka, Jaromír
http://linked.open.../riv/druhVysledku	G/B - Funkční vzorek
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...onomickeParametry	náklady v 300 000,- Kč, licenční poplatek 50 000,- Kč
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	316174
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/09:00337839
http://linked.open...terniIdentifikace	S. cerevisiae BYT4, BYT5 a BYT45
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open...vai/riv/kategorie	A - Do 5 mil. Kč
http://linked.open.../riv/klicovaSlova	yeast heterologous expression; S. cerevisiae; salt-sensitive mutant (en)
http://linked.open.../riv/klicoveSlovo	S. cerevisiae yeast heterologous expression salt-sensitive mutant
http://linked.open...ontrolniKodProRIV	[C729E39D3BF3]
http://linked.open.../licencniPoplatek	N - Poskytovatel licence na výsledek nepožaduje licenční poplatek
http://linked.open...okalizaceVysledku	Fyziologický ústav AVČR, v.v
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Alkali-metal-cation homeostasis in Saccharomyces cerevisiae biology Centre on molecular biology and physiology of yeast communities
http://linked.open...UplatneniVysledku	2009
http://linked.open...echnickeParametry	Mutantní kmeny S. cerevisiae BYT4, BYT5 a BYT45 s vysokou citlivostí k solím alkalických kovů jsou vhodné pro heterologní expresi transportérů sodíku a draslíku z jiných eukaryot
http://linked.open...iv/tvurceVysledku	Sychrová, Hana Zahrádka, Jaromír Herynková, Pavla
http://linked.open...avai/riv/vlastnik	Fyziologický ústav AVČR, v.v
http://linked.open...itiJinymSubjektem	P - Nabytí licence je nutné v některých případech
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka

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