About: Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata

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About: Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The ENA1 gene encoding plasma membrane Na+ATPase in the pathogenic C. glabrata was amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Ena1 proteins tagged with GFP at their C termini). Resulting plasmids allow the heterologous expression of Candida glabrata Ena1 transporter in S. cerevisiae strains and the study of its substrate specificity, activity and/or localization The ENA1 gene encoding plasma membrane Na+ATPase in the pathogenic C. glabrata was amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Ena1 proteins tagged with GFP at their C termini). Resulting plasmids allow the heterologous expression of Candida glabrata Ena1 transporter in S. cerevisiae strains and the study of its substrate specificity, activity and/or localization (en)
Title	Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata (en)
skos:prefLabel	Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata Set of multi-copy plasmids for heterologous expression and characterization of of transport properties and localization of Na+ ATPase from Candida glabrata (en)
skos:notation	RIV/67985823:_____/09:00337821!RIV10-MSM-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC531), Z(AV0Z50110509)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Krauke, Yannick Sychrová, Hana
http://linked.open.../riv/druhVysledku	G/B - Funkční vzorek
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...onomickeParametry	náklady 300 000,- Kč, licenční poplatek 50 000,- Kč
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	340936
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/09:00337821
http://linked.open...terniIdentifikace	CandidaENA1-YEP352/pGRU1
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open...vai/riv/kategorie	A - Do 5 mil. Kč
http://linked.open.../riv/klicovaSlova	Candida glabrata; plasma-membrane Na+ATPase; yeast heterologous expression (en)
http://linked.open.../riv/klicoveSlovo	plasma-membrane Na+ATPase Candida glabrata yeast heterologous expression
http://linked.open...ontrolniKodProRIV	[8639097B742D]
http://linked.open.../licencniPoplatek	N - Poskytovatel licence na výsledek nepožaduje licenční poplatek
http://linked.open...okalizaceVysledku	Fyziologický ústav AVČR, v.v
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre on molecular biology and physiology of yeast communities
http://linked.open...UplatneniVysledku	2009
http://linked.open...echnickeParametry	Sterilní vodné roztoky dvou plasmidových DNA určených pro transformaci E.coli (selekce AmpR) nebo S. cerevisiae (selekce Ura+) a počítačové soubory znázorňující sekvence DNA plasmidů
http://linked.open...iv/tvurceVysledku	Krauke, Yannick Sychrová, Hana
http://linked.open...avai/riv/vlastnik	Fyziologický ústav AVČR, v.v
http://linked.open...itiJinymSubjektem	P - Nabytí licence je nutné v některých případech
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka

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