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Description
  • The described software system for the coarse-to-fine positioning of XY motorized microscope stage allows us interactive control of image acquisition in confocal microscopy. An image captured through a low-resolution objective serves as navigation map where the user points to positions or areas of interest in which a high-resolution image will be captured. Our contribution presents an extension of a two-level coarse/fine algorithm to a multi-level environment. Moreover, the system is accompanied by algorithms that compensate both uneven illumination of neighbouring captured images (neighbouring fields of view) and light attenuation of confocal 3D images with depth, which are effects observed in confocal microscopy. By applying these algorithms resulting images of high quality are obtained. The system also enables us acquisition of confocal data sets for performing 3D reconstructions of large biological specimens
  • The described software system for the coarse-to-fine positioning of XY motorized microscope stage allows us interactive control of image acquisition in confocal microscopy. An image captured through a low-resolution objective serves as navigation map where the user points to positions or areas of interest in which a high-resolution image will be captured. Our contribution presents an extension of a two-level coarse/fine algorithm to a multi-level environment. Moreover, the system is accompanied by algorithms that compensate both uneven illumination of neighbouring captured images (neighbouring fields of view) and light attenuation of confocal 3D images with depth, which are effects observed in confocal microscopy. By applying these algorithms resulting images of high quality are obtained. The system also enables us acquisition of confocal data sets for performing 3D reconstructions of large biological specimens (en)
Title
  • A System for Automated Acquisition of 3D Biomedical Images Using Confocal Microscopy
  • A System for Automated Acquisition of 3D Biomedical Images Using Confocal Microscopy (en)
skos:prefLabel
  • A System for Automated Acquisition of 3D Biomedical Images Using Confocal Microscopy
  • A System for Automated Acquisition of 3D Biomedical Images Using Confocal Microscopy (en)
skos:notation
  • RIV/67985823:_____/09:00330404!RIV10-MSM-67985823
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA102/08/0691), P(GA304/09/0733), P(IAA100110502), P(LC06063), P(MEB080831), Z(AV0Z50110509), Z(MSM6840770012)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 301621
http://linked.open...ai/riv/idVysledku
  • RIV/67985823:_____/09:00330404
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • confocal laser scanning microscope; illumination inhomogeneity; automated acquisition system (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [9CB64C5948A2]
http://linked.open...v/mistoKonaniAkce
  • Graz
http://linked.open...i/riv/mistoVydani
  • Graz
http://linked.open...i/riv/nazevZdroje
  • MC 2009 : Microscopy Conference
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Janáček, Jiří
  • Kubínová, Lucie
  • Michálek, Jan
  • Čapek, Martin
  • Tomori, Z.
  • Hozman, J.
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Verlag der Technischen Universität
https://schema.org/isbn
  • 978-3-85125-062-6
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