About: Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithm to compensate for deformations due to cutting the specimen. We present a method enabling us to keep true object morphology using a-priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USB light microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithm to compensate for deformations due to cutting the specimen. We present a method enabling us to keep true object morphology using a-priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USB light microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance (en)
Title	Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration (en)
skos:prefLabel	Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration (en)
skos:notation	RIV/67985823:_____/09:00329247!RIV10-MSM-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA102/08/0691), P(GA304/09/0733), P(IAA100110502), P(LC06063), Z(AV0Z50110509), Z(MSM6840770012)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Brůža, Petr Čapek, Martin Janáček, Jiří Kubínová, Lucie Kocandová, Lucie
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	349876
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/09:00329247
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	3D reconstruction; elastic registration; 3D visualization; confocal microscopy (en)
http://linked.open.../riv/klicoveSlovo	elastic registration 3D reconstruction 3D visualization confocal microscopy
http://linked.open...ontrolniKodProRIV	[4BB5C42E603B]
http://linked.open...v/mistoKonaniAkce	Milano
http://linked.open...i/riv/mistoVydani	Bologna
http://linked.open...i/riv/nazevZdroje	Stereology and Image Analysis
http://linked.open...in/vavai/riv/obor	JC
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Visualization and processing of 3D images captured by a confocal microscope using graphics processor unit programming and virtual reality Three-dimensional arrangement and remodelation of the capillary bed of placentas from pregnancies complicated by type I diabetes mellitus Flurescence microscopy in biological and medical research Spatial statistics of 3D confocal images of capillary network
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Janáček, Jiří Kubínová, Lucie Čapek, Martin Brůža, Petr Kocandová, Lucie Kneppo, P. Hozman, J. Vagnerová, R.
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2009-06-22 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Transdisciplinární výzkum v oblasti biomedicínského inženýrství II
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Esculapio
https://schema.org/isbn	978-88-7488-310-3

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