| Attributes | Values |
|---|
| rdf:type
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| Description
| - Geny CNH1 kódující homologní Na+/H+-antiportní systémy plasmatické membrány z patogenních kvasinek C. albicans, C. glabrata, C. parapsilosis nebo C. dubliniensis byly amplifikovány PCR a klonovány za konstitutivní promotor genu NHA1 ze Saccharomyces cerevisiae do člunkových vektorů S. cerevisiae/E.coli (URA3/AmpR) YEp352 nebo pGRU1 (pro expresi proteinů Cnh1 značených GFP na C konci). Výsledných 8 plasmidů umožňuje heterologní expresi antiporterů Cnh1 z Candida sp. v kmenech S. cerevisiae a tím i studium a porovnání jejich substratové specifity, transportní aktivity nebo lokalizace v buňkách (cs)
- The CNH1 genes encoding homologous plasma membrane Na+/H+ antiporters from pathogenic C. albicans, C. glabrata, C. parapsilosis or C. dubliniensis were amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Cnh1 proteins tagged with GFP at their C termini). Resulting 8 plasmids allow the heterologous expression of Candida sp. Cnh1 antiporters in S. cerevisiae strains and the study and comparison of their substrate specificity, activity or localization
- The CNH1 genes encoding homologous plasma membrane Na+/H+ antiporters from pathogenic C. albicans, C. glabrata, C. parapsilosis or C. dubliniensis were amplified by PCR and cloned behind the constitutive Saccharomyces cerevisiae NHA1 promotor into S. cerevisiae/E.coli (URA3/AmpR) shuttle vectors YEp352 or pGRU1 (for expression of Cnh1 proteins tagged with GFP at their C termini). Resulting 8 plasmids allow the heterologous expression of Candida sp. Cnh1 antiporters in S. cerevisiae strains and the study and comparison of their substrate specificity, activity or localization (en)
|
| Title
| - Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)
- Sada mnohokopiových plasmidů pro studium transportních vlastností a lokalizace Na+/H+-antiportních systémů ze 4 patogenních druhů kvasinek rodu Candida (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis) (cs)
- Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis) (en)
|
| skos:prefLabel
| - Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis)
- Sada mnohokopiových plasmidů pro studium transportních vlastností a lokalizace Na+/H+-antiportních systémů ze 4 patogenních druhů kvasinek rodu Candida (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis) (cs)
- Set of multi-copy plasmids for the study of transport properties and localization of Na+/H+ antiporters from four patogenic Candida yeast species (C. albicans, C. glabrata, C. parapsilosis, C. dubliniensis) (en)
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| skos:notation
| - RIV/67985823:_____/08:00324265!RIV09-AV0-67985823
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(KJB5011307), P(LC531), Z(AV0Z50110509)
|
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...onomickeParametry
| - náklady 300 000,- Kč, licenční poplatek 50 000,- Kč
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/67985823:_____/08:00324265
|
| http://linked.open...terniIdentifikace
| |
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open...vai/riv/kategorie
| |
| http://linked.open.../riv/klicovaSlova
| - Candida sp.; plasma-membrane Na+/H+ antiporter; heterologous expression (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
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| http://linked.open.../licencniPoplatek
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| http://linked.open...okalizaceVysledku
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...echnickeParametry
| - Sterilní vodné roztoky osmi plasmidových DNA určených pro transformaci E.coli (selekce AmpR) nebo S. cerevisiae (selekce Ura+) a tři počítačové soubory znázorňující sekvence DNA plasmidů
|
| http://linked.open...iv/tvurceVysledku
| - Krauke, Yannick
- Sychrová, Hana
- Kinclová, Olga
|
| http://linked.open...avai/riv/vlastnik
| |
| http://linked.open...itiJinymSubjektem
| |
| http://linked.open...n/vavai/riv/zamer
| |
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