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Description
  • We tested different standards, such as calibration procedures based on uniform thin fluorescent layer or fluorescent microspheres applied to calibration of 1PE as well as 2PE microscope systems. We compared the different types of calibration measurements with the aim to find a reliable and easy-to-use tool for routine checking of the system. We found out that some very simple samples, such as usual coverslip can be useful for basic checking of system setting and performance, however, for proper calibration of the confocal systems usually a combination of several more complex calibration measurements is necessary
  • We tested different standards, such as calibration procedures based on uniform thin fluorescent layer or fluorescent microspheres applied to calibration of 1PE as well as 2PE microscope systems. We compared the different types of calibration measurements with the aim to find a reliable and easy-to-use tool for routine checking of the system. We found out that some very simple samples, such as usual coverslip can be useful for basic checking of system setting and performance, however, for proper calibration of the confocal systems usually a combination of several more complex calibration measurements is necessary (en)
  • Testovali jsme různé kalibrační postupy pro jedno- i dvoufotonovou mikroskopii, např. s využitím vzorku s rovnoměrnou tenkou fluorescenční vrstvou či fluorescenčních mikrokuliček. Porovnávali jsme různé typy kalibračních měření s cílem najít spolehlivý a snadno použitelný nástroj pro rutinní kontrolu systému. Zjistili jsme, že některé velmi jednoduché vzorky, jako obyčejné krycí sklo, mohou být pro základní testování použitelné, avšak pro správnou kalibraci konfokálních systémů je zpravidla zapotřebí udělat více složitějších kalibračních měření (cs)
Title
  • Testing calibration standarts for confocal and two-photon microscopy
  • Testování kalibračních vzorků pro konfokální a dvoufotonovou mikroskopii (cs)
  • Testing calibration standarts for confocal and two-photon microscopy (en)
skos:prefLabel
  • Testing calibration standarts for confocal and two-photon microscopy
  • Testování kalibračních vzorků pro konfokální a dvoufotonovou mikroskopii (cs)
  • Testing calibration standarts for confocal and two-photon microscopy (en)
skos:notation
  • RIV/67985823:_____/08:00318206!RIV09-AV0-67985823
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAA500200510), P(LC06063), Z(AV0Z50110509)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 399668
http://linked.open...ai/riv/idVysledku
  • RIV/67985823:_____/08:00318206
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • confocal microscopy; calibration; two-photon microscopy (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [33A78561AB4D]
http://linked.open...v/mistoKonaniAkce
  • Aachen
http://linked.open...i/riv/mistoVydani
  • Berlin
http://linked.open...i/riv/nazevZdroje
  • EMC 2008
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Kubínová, Lucie
  • Chernyavskiy, Oleksandr
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Springer-Verlag
https://schema.org/isbn
  • 978-3-540-85227-8
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