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Description
  • V této práci je popsána technika pro rychlé chlazení a zahřívání buněk v tkáňové kultuře. Princip tohoto systému spočívá v zpětnovazebné kontrole teploty roztoku omývajícího buňky při experimentu. Tato technika je vhodná pro studium teplotní závislosti iontových kanálů. Práce je doprovázena příklady využití techniky pro teplotně aktivované kanály TRPV1, TRPM8 heterologně exprimované v HEK buňkách a sensorické neurony pěstované v kultuře (cs)
  • This work comprises characterization of the technique used for rapid cooling and heating of the cells in tissue culture. Principle of the technique is feedback control of temperature of the solution superfusing the cells during the experiment. This system is suitable for the study of temperature dependence of ion channels. The work is accompanied with examples of use of the technique for temperature-activated channels TRPV1, TRPM8 heterologously expressed in HEK cells and sensory neurons in tissue culture
  • This work comprises characterization of the technique used for rapid cooling and heating of the cells in tissue culture. Principle of the technique is feedback control of temperature of the solution superfusing the cells during the experiment. This system is suitable for the study of temperature dependence of ion channels. The work is accompanied with examples of use of the technique for temperature-activated channels TRPV1, TRPM8 heterologously expressed in HEK cells and sensory neurons in tissue culture (en)
Title
  • Improved superfusion technique for rapid cooling or heating of cultured cells under patch-clamp conditions
  • Zdokonalená superfuzní technika pro rychlé chlazení a zahřívání buněk v tkáňové kultuře pro použití v patch-clamp experimentech (cs)
  • Improved superfusion technique for rapid cooling or heating of cultured cells under patch-clamp conditions (en)
skos:prefLabel
  • Improved superfusion technique for rapid cooling or heating of cultured cells under patch-clamp conditions
  • Zdokonalená superfuzní technika pro rychlé chlazení a zahřívání buněk v tkáňové kultuře pro použití v patch-clamp experimentech (cs)
  • Improved superfusion technique for rapid cooling or heating of cultured cells under patch-clamp conditions (en)
skos:notation
  • RIV/67985823:_____/06:00039248!RIV07-AV0-67985823
http://linked.open.../vavai/riv/strany
  • 178;185
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1M0517), P(GA305/03/0802), P(LC554), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 479072
http://linked.open...ai/riv/idVysledku
  • RIV/67985823:_____/06:00039248
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • capsaicin; TRPV1; TRPM8 (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [54D01859FE94]
http://linked.open...i/riv/nazevZdroje
  • Journal of Neuroscience Methods
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 151
http://linked.open...iv/tvurceVysledku
  • Benedikt, Jan
  • Vlachová, Viktorie
  • Vyklický st., Ladislav
  • Reeh, P. W.
  • Zimmermann, K.
  • Dittert, Ivan
http://linked.open...n/vavai/riv/zamer
issn
  • 0165-0270
number of pages
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