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rdf:type
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Description
| - Mutants were constructed for mitochondrial uncoupling protein UCP1, with single or multiple substitutions within or nearby the UCP-signatures located in the 1st alfa-helix and 2nd matrix segment, using the QuickChange site directed mutagenesis protocol (Stratagene), and were assayed fluorometrically for kinetics of fatty acid (FA)-induced H+ uniport and for Cl- uniport. Their ability to bind 3H-GTP was also evaluated. The wild type UCP1 was associated with the FA-induced H+ uniport proportional to the added protein with a Km for lauric acid of 43 mikroM and Vmax of 18 mikromol.min-1.(mg protein)-1. Neutralization of Arg 152 (in the 2nd matrix segment UCP-signature) led to ~50% reduction of FA affinity (reciprocal Km) and of Vmax for FA-induced H+ uniport. Halved FA-affinity and 70% reduction of Vmax was found for the double His substitution outside the signature (H145L & H147L mutant). Neutralization of Asp 27 in the1st alfa-helix UCP-signature (D27V mutant) resulted in 75% reduction of FA affinity an
- Mutants were constructed for mitochondrial uncoupling protein UCP1, with single or multiple substitutions within or nearby the UCP-signatures located in the 1st alfa-helix and 2nd matrix segment, using the QuickChange site directed mutagenesis protocol (Stratagene), and were assayed fluorometrically for kinetics of fatty acid (FA)-induced H+ uniport and for Cl- uniport. Their ability to bind 3H-GTP was also evaluated. The wild type UCP1 was associated with the FA-induced H+ uniport proportional to the added protein with a Km for lauric acid of 43 mikroM and Vmax of 18 mikromol.min-1.(mg protein)-1. Neutralization of Arg 152 (in the 2nd matrix segment UCP-signature) led to ~50% reduction of FA affinity (reciprocal Km) and of Vmax for FA-induced H+ uniport. Halved FA-affinity and 70% reduction of Vmax was found for the double His substitution outside the signature (H145L & H147L mutant). Neutralization of Asp 27 in the1st alfa-helix UCP-signature (D27V mutant) resulted in 75% reduction of FA affinity an (en)
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Title
| - Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport.
- Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport. (en)
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skos:prefLabel
| - Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport.
- Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H(+) uniport. (en)
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skos:notation
| - RIV/67985823:_____/03:20030011!RIV/2004/GA0/A20004/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA301/02/1215), P(GA301/98/0568), P(IAA5011106), Z(AV0Z5011922)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/67985823:_____/03:20030011
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - site-directed mutagenesis; mitochondrial uncoupling protein-1; fatty acid-inducted H+ uniport (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - International Journal of Biochemistry & Cell Biology
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Ježek, Petr
- Růžička, Michal
- Hanák, Petr
- Urbánková, Eva
- Škobisová, Eva
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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