About: Thermal stability of the human blood serum acid alpha(1)-glycoprotein in acidic media.

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About: Thermal stability of the human blood serum acid alpha(1)-glycoprotein in acidic media. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Thermal stability of human alfa(1)-acid glycoprotein and its desialyzed form were studied in the pH range 1.5-5.2, i.e. about its pI. Circular dichroism, fluorescence, and UV-absorption were used to determine the conformational changes and their reversibility in the temperature range 25-80 C. These changes were tested in a three step process - heating, cooling, and a second heating. Principal component analysis was applied for analyzing the spectral sets obtained in these experiments. Fully reversible behavior of Trp residues, as characterized by fluorescence spectroscopy, was observed during the heating process at all pH values. Nevertheless, three different types of the protein motion (reversible, irreversible, and rearrangement of the protein core) were determined by UV-absorption spectroscopy. Thus, an environment of Tyr and Phe is modified or reversibly rearranged during the heating process in acid media. These types of alfa(1)-acid glycoprotein behavior were not significantly affected by desialy Thermal stability of human alfa(1)-acid glycoprotein and its desialyzed form were studied in the pH range 1.5-5.2, i.e. about its pI. Circular dichroism, fluorescence, and UV-absorption were used to determine the conformational changes and their reversibility in the temperature range 25-80 C. These changes were tested in a three step process - heating, cooling, and a second heating. Principal component analysis was applied for analyzing the spectral sets obtained in these experiments. Fully reversible behavior of Trp residues, as characterized by fluorescence spectroscopy, was observed during the heating process at all pH values. Nevertheless, three different types of the protein motion (reversible, irreversible, and rearrangement of the protein core) were determined by UV-absorption spectroscopy. Thus, an environment of Tyr and Phe is modified or reversibly rearranged during the heating process in acid media. These types of alfa(1)-acid glycoprotein behavior were not significantly affected by desialy (en)
Title	Thermal stability of the human blood serum acid alpha(1)-glycoprotein in acidic media. Thermal stability of the human blood serum acid alpha(1)-glycoprotein in acidic media. (en)
skos:prefLabel	Thermal stability of the human blood serum acid alpha(1)-glycoprotein in acidic media. Thermal stability of the human blood serum acid alpha(1)-glycoprotein in acidic media. (en)
skos:notation	RIV/67985823:_____/03:20030003!RIV/2004/AV0/A20004/N
http://linked.open.../vavai/riv/strany	25;33
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z5011922), Z(MSM 113200002)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Kopecký, Vladimír Hofbauerová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	630920
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/03:20030003
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	orosomucoid; thermal stability; UV-spectroscopy (en)
http://linked.open.../riv/klicoveSlovo	orosomucoid thermal stability UV-spectroscopy
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[739BB873EE0B]
http://linked.open...i/riv/nazevZdroje	Biophysical Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	103
http://linked.open...iv/tvurceVysledku	Hofbauerová, Kateřina Karpenko, V. Sýkora, J. Kopecký jr., Vladimír
http://linked.open...n/vavai/riv/zamer	Investigation of normal and pathological physiology of animals - the clarification of the physiological mechanisms operating in humans in health and disease http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20113200002
issn	0301-4622
number of pages	9 (xsd:int)

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