About: Determination of anti-acetylcholine receptor antibodies in myasthenic patients by use of time-resolved fluorescence.

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Determination of anti-acetylcholine receptor antibodies in myasthenic patients by use of time-resolved fluorescence. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Autoantibodies against nicotinic acetylcholine receptor (nAChR) in myasthenia gravis (MG) patients are usually detected by radioimmunoprecipitation assays using extracted acetylcholine receptors labeled irreversibly with 125I-alpha-bungarotoxin (alpha-BuTx). We established an assay using nAChRs labeled with Eu(3+)-alpha-cobratoxin (alpha-CTx). Eu(3+)-labeled alpha-CTx competed with 125I- alpha-BuTx for binding to Torpedo nAChRs and saturated the binding sites of human nAChRs, with a K(d) of 7.2 x 10(-9) mol/L. Results of the immunoassay performed with Eu(3+)-labeled alpha-CTx were similar to those obtained with 125I-alpha-BuTx, with a slightly higher limit of detection [0.3 nmol/L (n = 6) vs approximately 0.1 nmol/L for isotopic assay]. None of 34 negative sera tested gave a value >0.3 nmol/L. Of the 35 positive myasthenic sera (with antibody values, previously determined by isotopic assay, of 0.4- 1290 nmol/L) compared in the two assays, 32 tested positive with the Eu(3+) assay. Linear regression ana Autoantibodies against nicotinic acetylcholine receptor (nAChR) in myasthenia gravis (MG) patients are usually detected by radioimmunoprecipitation assays using extracted acetylcholine receptors labeled irreversibly with 125I-alpha-bungarotoxin (alpha-BuTx). We established an assay using nAChRs labeled with Eu(3+)-alpha-cobratoxin (alpha-CTx). Eu(3+)-labeled alpha-CTx competed with 125I- alpha-BuTx for binding to Torpedo nAChRs and saturated the binding sites of human nAChRs, with a K(d) of 7.2 x 10(-9) mol/L. Results of the immunoassay performed with Eu(3+)-labeled alpha-CTx were similar to those obtained with 125I-alpha-BuTx, with a slightly higher limit of detection [0.3 nmol/L (n = 6) vs approximately 0.1 nmol/L for isotopic assay]. None of 34 negative sera tested gave a value >0.3 nmol/L. Of the 35 positive myasthenic sera (with antibody values, previously determined by isotopic assay, of 0.4- 1290 nmol/L) compared in the two assays, 32 tested positive with the Eu(3+) assay. Linear regression ana (en)
Title	Determination of anti-acetylcholine receptor antibodies in myasthenic patients by use of time-resolved fluorescence. Determination of anti-acetylcholine receptor antibodies in myasthenic patients by use of time-resolved fluorescence. (en)
skos:prefLabel	Determination of anti-acetylcholine receptor antibodies in myasthenic patients by use of time-resolved fluorescence. Determination of anti-acetylcholine receptor antibodies in myasthenic patients by use of time-resolved fluorescence. (en)
skos:notation	RIV/67985823:_____/02:20020034!RIV/2003/AV0/A20003/N
http://linked.open.../vavai/riv/strany	549;554
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(NF4646), Z(AV0Z5011922)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Říčný, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	642697
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/02:20020034
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nicotinic acetylcholine receptor; time-resolved fluorescence method; myasthenia gravis (en)
http://linked.open.../riv/klicoveSlovo	nicotinic acetylcholine receptor time-resolved fluorescence method myasthenia gravis
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[17FD286CE751]
http://linked.open...i/riv/nazevZdroje	Clinical Chemistry
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	New method for determination of antibodies against nicotinic acetylcholine receptors in patients with myasthenia gravis.
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Říčný, Jan Šimková, L. Vincent, A.
http://linked.open...n/vavai/riv/zamer	Investigation of normal and pathological physiology of animals - the clarification of the physiological mechanisms operating in humans in health and disease
issn	0009-9147
number of pages	6 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software