About: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fungal b-N-acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship with other fungal hexosaminidases. It has high GalNAcase activity, tolerates substrates with the modified N-acyl group better and has some other unusual catalytic properties. In order to understand these features, we performed isolation, biochemical and enzymological characterization, molecular cloning and molecular modelling. The native enzyme is composed of two catalytic units (65 kDa each) and two propeptides (15 kDa each), yielding a molecular weight of 160 kDa Fungal b-N-acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship with other fungal hexosaminidases. It has high GalNAcase activity, tolerates substrates with the modified N-acyl group better and has some other unusual catalytic properties. In order to understand these features, we performed isolation, biochemical and enzymological characterization, molecular cloning and molecular modelling. The native enzyme is composed of two catalytic units (65 kDa each) and two propeptides (15 kDa each), yielding a molecular weight of 160 kDa (en)
Title	Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase (en)
skos:prefLabel	Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase (en)
skos:notation	RIV/67179843:_____/11:00366613!RIV12-AV0-67179843
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M0505), P(GA303/09/0477), P(GD305/09/H008), P(GP203/09/P024), P(LC06010), S, Z(AV0Z50200510), Z(AV0Z60870520), Z(MSM0021620808), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kulik, Natallia Ettrich, Rüdiger
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	197794
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/11:00366613
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	deglycosylation; enzyme kinetics; hexosaminidase (en)
http://linked.open.../riv/klicoveSlovo	deglycosylation enzyme kinetics hexosaminidase
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FB0761E83D9D]
http://linked.open...i/riv/nazevZdroje	FEBS Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	14 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of targeted therapeutic drugs Preparation, biotransformation, and optimalization of compounds with antitumor and antimicrobial effects Center of Biocatalysis and Biotransformation Glycomic and proteomic analysis of the molecular mechanisms of elimination of clinically relevant tumors by killer cells of the immune system Synthesis of immunoactive glycosides by beta-N-acetylhexosaminidases
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	278
http://linked.open...iv/tvurceVysledku	Bezouška, Karel Bojarová, Pavla Ettrich, Rüdiger Kulik, Natallia Křen, Vladimír Kumar, V. Slámová, Kristýna Vaněk, Ondřej Doubnerová, V. Kalendová, A. Kukačka, Z. Pompach, Petr Ryšlavá, H. Skočdopol, P.
http://linked.open...ain/vavai/riv/wos	000292932700007
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	1742-464X
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/j.1742-4658.2011.08173.x

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