About: The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase

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About: The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two genes in the genome of Aspergillus niger, aglA and aglB, have been assigned to encode for α-d-galactosidases variant A and B. However, analyses of primary and 3D structures based on structural models of these two enzymes revealed significant differences in their active centers suggesting important differences in their specificity for the hydrolyzed carbohydrates. To test this unexpected finding, a large screening of libraries from 42 strains of filamentous fungi succeeded in identifying an enzyme from A. niger CCIM K2 that exhibited both α-galactosidase andα-N-acetylgalactosaminidase activities, with the latter activity predominating. The enzyme protein was sequenced, and its amino acid sequence could be unequivocally assigned to the enzyme encoded the aglA gene. Enzyme activity measurements and substrate docking clearly demonstrated the preference of the identified enzyme for α-N-acetyl-d-galactosaminide over α-d-galactoside. Two genes in the genome of Aspergillus niger, aglA and aglB, have been assigned to encode for α-d-galactosidases variant A and B. However, analyses of primary and 3D structures based on structural models of these two enzymes revealed significant differences in their active centers suggesting important differences in their specificity for the hydrolyzed carbohydrates. To test this unexpected finding, a large screening of libraries from 42 strains of filamentous fungi succeeded in identifying an enzyme from A. niger CCIM K2 that exhibited both α-galactosidase andα-N-acetylgalactosaminidase activities, with the latter activity predominating. The enzyme protein was sequenced, and its amino acid sequence could be unequivocally assigned to the enzyme encoded the aglA gene. Enzyme activity measurements and substrate docking clearly demonstrated the preference of the identified enzyme for α-N-acetyl-d-galactosaminide over α-d-galactoside. (en)
Title	The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase (en)
skos:prefLabel	The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase The alpha-galactosidase type A gene aglA from Aspergillus niger encodes a fully functional alpha-N-acetylgalactosaminidase (en)
skos:notation	RIV/67179843:_____/10:00355844!RIV11-GA0-67179843
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA303/09/0477), P(GAP207/10/0321), P(GAP207/10/1040), P(GAP207/10/1934), P(GD305/09/H008), P(LC06010), S, Z(AV0Z10750506), Z(AV0Z50200510), Z(AV0Z60870520), Z(MSM0021620808), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kulik, Natallia Ettrich, Rüdiger
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	246010
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/10:00355844
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	α-N-acetylgalactosaminidase; α-galactosidase; Aspergillus niger; substrate binding; molecular modeling (en)
http://linked.open.../riv/klicoveSlovo	Aspergillus niger molecular modeling α-N-acetylgalactosaminidase α-galactosidase substrate binding
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[21881B53448F]
http://linked.open...i/riv/nazevZdroje	Glycobiology
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Preparation, biotransformation, and optimalization of compounds with antitumor and antimicrobial effects Center of Biocatalysis and Biotransformation Structural insight into E. coli protein WrbA, the founding member of a family of proteins implicated in defense of cells against oxidative stress Glycomic and proteomic analysis of the molecular mechanisms of elimination of clinically relevant tumors by killer cells of the immune system The ways to N-acetyl-mannosamine structures via mannoside metabolizing enzymes Analysis of protein complexes of killer cell receptors using chemical cross-linking, surface labeling, isotope exchange, mass spectrometry and NMR.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	20
http://linked.open...iv/tvurceVysledku	Ettrich, Rüdiger Kulik, Natallia Křen, Vladimír Novák, Petr Slámová, Kristýna Pompach, Petr Filipi, Tomáš Bezouška, K. Mrázek, H. Weignerová, L.
http://linked.open...ain/vavai/riv/wos	000282750300009
http://linked.open...n/vavai/riv/zamer	Pokročilé matematické metody získávání, zpracování a využití informací a znalostí ve složitých a nedeterministických systémech Microorganisms in Research and Biotechnology Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Molekulárně biologické, genetické a epigenetické aspekty vzniku a rozvoje modelových tumorů dospělého věku. Význam pro epidemiologii, časnou diagnostiku a léčbu Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	0959-6658
number of pages	10 (xsd:int)

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