About: Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water

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About: Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this work was to modify the method of Ladd and Buttler (1972), by substituting Tris–HCl buffer (pH 8.52) with demineralized water (DEMI H2O), in order to assess its suitability for measurement of casein-protease activity at pH levels close to those of real soil in H2O. The substitution of Tris–HCl buffer by DEMI H2O at 49°C decreased casein-protease activity to 67.25% in mineral soil and to 53.76% in organic soil. With decreasing temperature casein-protease activity decreased the most in organic soil. The incubation period was extended to maximally 336 h at 3°C to totally obtain >10.0% of L-tyrosine equivalents released at optimum temperature and pH conditions. The Q10 values of casein-protease activity measured after substituting Tris–HCl buffer with DEMI H2O were unexpectedly high. Between the temperatures of 3 and 49°C Q10 ranged from 3.46 to 4.25, whereas between 3 and 25°C Q10 ranged from 6.78 to 11.08. The aim of this work was to modify the method of Ladd and Buttler (1972), by substituting Tris–HCl buffer (pH 8.52) with demineralized water (DEMI H2O), in order to assess its suitability for measurement of casein-protease activity at pH levels close to those of real soil in H2O. The substitution of Tris–HCl buffer by DEMI H2O at 49°C decreased casein-protease activity to 67.25% in mineral soil and to 53.76% in organic soil. With decreasing temperature casein-protease activity decreased the most in organic soil. The incubation period was extended to maximally 336 h at 3°C to totally obtain >10.0% of L-tyrosine equivalents released at optimum temperature and pH conditions. The Q10 values of casein-protease activity measured after substituting Tris–HCl buffer with DEMI H2O were unexpectedly high. Between the temperatures of 3 and 49°C Q10 ranged from 3.46 to 4.25, whereas between 3 and 25°C Q10 ranged from 6.78 to 11.08. (en)
Title	Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water (en)
skos:prefLabel	Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water Estimation of protease activity in soils at low temperatures by casein amendment and with substitution of buffer by demineralized water (en)
skos:notation	RIV/67179843:_____/08:00341974!RIV10-MZP-67179843
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(SM/640/18/03), Z(MSM6215648902)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pavelka, Marian
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	366552
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/08:00341974
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Soil proteases; Enzyme temperature dependence; Q10 (en)
http://linked.open.../riv/klicoveSlovo	Soil proteases Q10 Enzyme temperature dependence
http://linked.open...odStatuVydavatele	AT - Rakouská republika
http://linked.open...ontrolniKodProRIV	[A968CF472CDD]
http://linked.open...i/riv/nazevZdroje	Amino Acids
http://linked.open...in/vavai/riv/obor	DF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	CzechCarbo - Carbon cycle study focused on the terrestrical ecosystems in the Czech republic in connection on the CARBOEUROPE project
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	35
http://linked.open...iv/tvurceVysledku	Formánek, P. Pavelka, Marian Rejšek, K.
http://linked.open...n/vavai/riv/zamer	Forest and Wood: the support of functionally integrated forest management and use of wood as a renewable raw material
issn	0939-4451
number of pages	7 (xsd:int)

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