| Attributes | Values |
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| rdf:type
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| Description
| - Tato práce porovnává použití polymerasové řetězové reakce (PCR) se standardní kultivační metodou CSN/ISO/10272 pro stanovení Campylobacter spp. v potravinách obsahujících kuřečí maso a určených k přímé spotřebě. Pro PCR se použily primery specifické pro C. jejuni, C. coli, C. lari a interní kontrola. Stanovení kampylobakterů bylo prováděno jak v neošetřených vzorcích tak také v uměle kontaminovaných vzorcích potravin zakoupených v maloobchodní síti. Detekční limit PCR byl 2 KTJ/g po 48h kultivaci v bujónu Parka a Sanderse. Duplex PCR se ukázal být vysoce spolehlivý pro stanovení kampylobakterů v různých typech potravin. I bez umělé kontaminace vykázaly vzorky z celosvětového řetězce s rychlým občerstvením po PCR amplifikaci pozitivní produkt zatímco výsledky kultivací stejných vzorků byly negativní. (cs)
- This work compared the use of polymerase chain reaction (PCR) and the conventional CSN/ISO/10272 culture-based methods in the detection of Campylobacter species in ready-to-eat meals made from chicken meat. PCR was carried out with the primers specific to C. jejuni, C. coli, C. lari, and was modified with an internal control. The detection of campylobacters by PCR was performed on both untreated and spiked samples of real food purchased in local stores. For PCR, the detection limit was 2 CFU/g after 48 h enrichment in Park and Sanders broth. Duplex PCR proved to be highly reliable in the detection of campylobacters in different food types. Without extra spiking, samples from a global fast food chain exhibited positive amplification of the PCR product while but negative results were obtained from the cultivation of the same samples.
- This work compared the use of polymerase chain reaction (PCR) and the conventional CSN/ISO/10272 culture-based methods in the detection of Campylobacter species in ready-to-eat meals made from chicken meat. PCR was carried out with the primers specific to C. jejuni, C. coli, C. lari, and was modified with an internal control. The detection of campylobacters by PCR was performed on both untreated and spiked samples of real food purchased in local stores. For PCR, the detection limit was 2 CFU/g after 48 h enrichment in Park and Sanders broth. Duplex PCR proved to be highly reliable in the detection of campylobacters in different food types. Without extra spiking, samples from a global fast food chain exhibited positive amplification of the PCR product while but negative results were obtained from the cultivation of the same samples. (en)
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| Title
| - Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals
- Optimalizace PCR metody pro stanovení Campylobacter jejuni a Campylobacter coli ve vzorcích potravin obsahujících kuřecí maso a určených k přímé spotřebě (cs)
- Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals (en)
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| skos:prefLabel
| - Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals
- Optimalizace PCR metody pro stanovení Campylobacter jejuni a Campylobacter coli ve vzorcích potravin obsahujících kuřecí maso a určených k přímé spotřebě (cs)
- Optimisation of the PCR Method for the Detection of Campylobacter jejuni and Campylobacter coli in Samples of Ready-to-Eat Chicken Meals (en)
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| skos:notation
| - RIV/67179843:_____/08:00312083!RIV09-AV0-67179843
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - Z(AV0Z60870520), Z(MSM6046137305)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/67179843:_____/08:00312083
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - polymerase chain reaction; internal control; Campylobacter spp. (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Czech Journal of Food Science
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Pazlarová, J.
- Demnerová, K.
- Šabatková, Zdeňka
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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