About: Magnetic ovalbumin and egg white aggregates as affinity adsorbents for lectins separation     Goto   Sponge   NotDistinct   Permalink

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Description
  • Byla vyvinuta jednoduchá a levná příprava nových magnetckých adsorbentů z ovalbuminu a vaječného bílku založená na precipitaci metanolem s následným prokřížením glutaraldehydem. Adsorbenty byly použity pro zakoncentrování dvou rostliných lektinů z bramborové hlízy (Solanum tuberosum) a z pšeničného klíčku (Triticum spp.). Zachycené lektiny byly eluovány ředěnou kyselinou chlorovodíkovou. Specifická aktivita obou lektinů vzrostla v přůběhu koncentračního procesu přibližně 30 – 40krát. (cs)
  • Simple and low-cost preparation of new magnetic adsorbents from ovalbumin and egg white, based on methanol precipitation and subsequent glutaraldehyde cross-linking has been developed. These adsorbents were used for preconcentration of two plant lectins from potato (Solanum tuberosum) tubers and from wheat (Triticum spp.) germs extracts. The adsorbed lectins were eluted with diluted hydrochloric acid. The specific activities of both lectins increased approximately 30–40 times during the preconcentration process.
  • Simple and low-cost preparation of new magnetic adsorbents from ovalbumin and egg white, based on methanol precipitation and subsequent glutaraldehyde cross-linking has been developed. These adsorbents were used for preconcentration of two plant lectins from potato (Solanum tuberosum) tubers and from wheat (Triticum spp.) germs extracts. The adsorbed lectins were eluted with diluted hydrochloric acid. The specific activities of both lectins increased approximately 30–40 times during the preconcentration process. (en)
Title
  • Magnetic ovalbumin and egg white aggregates as affinity adsorbents for lectins separation
  • Magnetic ovalbumin and egg white aggregates as affinity adsorbents for lectins separation (en)
  • Magnetické agregáty ovalbuminu a vaječného bílku jako afinitní sorbenty pro separaci lektinů (cs)
skos:prefLabel
  • Magnetic ovalbumin and egg white aggregates as affinity adsorbents for lectins separation
  • Magnetic ovalbumin and egg white aggregates as affinity adsorbents for lectins separation (en)
  • Magnetické agregáty ovalbuminu a vaječného bílku jako afinitní sorbenty pro separaci lektinů (cs)
skos:notation
  • RIV/67179843:_____/08:00309615!RIV09-AV0-67179843
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2A-1TP1/094), P(OC 157), Z(AV0Z60870520), Z(MSM6198959218)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 377415
http://linked.open...ai/riv/idVysledku
  • RIV/67179843:_____/08:00309615
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • egg white; lectins; magnetic protein aggregates (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [1A18441A8AB8]
http://linked.open...i/riv/nazevZdroje
  • Biochemical Engineering Journal
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 40
http://linked.open...iv/tvurceVysledku
  • Šafařík, Ivo
  • Šafaříková, Miroslava
  • Šabatková, Zdeňka
http://linked.open...ain/vavai/riv/wos
  • 000257536600020
http://linked.open...n/vavai/riv/zamer
issn
  • 1369-703X
number of pages
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