About: Crystallization of the flavoprotein WrbA optimized by using additives and gels

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	he tryptophan (W)-repressor binding protein A (WrbA) identified as an Escherichia coli stationary-phase protein was proposed as the founding member of a new family of multimeric flavodoxin-like proteins implicated in oxidative-stress defense. Since WrbA is largely uncharacterized with respect to both molecular and physiological functions, the present effort is aimed at structural characterization. WrbA apoprotein was purified and used for crystallization trials at room temperature. Multicrystals of WrbA apoprotein were obtained using standard and advanced crystallization techniques. Application of additives and gelling protein for crystallization in single capillaries yielded diffraction-quality single crystals. The crystals diffracted to a resolution of 2.2 angstrom at synchrotrons DESY (X13) in Hamburg (Germany), and Elletra (XRD1) in Trieste (Italy). (c) 2005 Elsevier B.V. All rights reserved. he tryptophan (W)-repressor binding protein A (WrbA) identified as an Escherichia coli stationary-phase protein was proposed as the founding member of a new family of multimeric flavodoxin-like proteins implicated in oxidative-stress defense. Since WrbA is largely uncharacterized with respect to both molecular and physiological functions, the present effort is aimed at structural characterization. WrbA apoprotein was purified and used for crystallization trials at room temperature. Multicrystals of WrbA apoprotein were obtained using standard and advanced crystallization techniques. Application of additives and gelling protein for crystallization in single capillaries yielded diffraction-quality single crystals. The crystals diffracted to a resolution of 2.2 angstrom at synchrotrons DESY (X13) in Hamburg (Germany), and Elletra (XRD1) in Trieste (Italy). (c) 2005 Elsevier B.V. All rights reserved. (en) Protein WrbA (tryptophan (W)-repressor binding protein A) identifikovaný jako protein stacionární části E.coli byl navržen jako zakládající člen nové rodiny multimerních proteinů podobných flavodoxinům. Protože je WrbA do značné míry necharakterizovaný s ohledem na jeho molekulární i fyziologickou funkci, součastné úsilí je zaměřeno na jeho strukturní studie. WrbA apoprotein byl purifikován a použit pro krystalizační experimenty prováděné při laboratorní teplotě. Multikrystaly WrbA apoproteinu byly získány použitím standardních a pokročilých krystalizačních technik. Použití aditiv a gelace proteinu při krystalizaci v kapilárách vedly k získání difraktujících monokrystalů. Krystaly difraktovaly do rozlišení 2.2? a byly měřeny na synchrotronech DESY (X13) v Hamburku (Germany) a Elletra (XRD1) v Terstu (Italy). (cs)
Title	Crystallization of the flavoprotein WrbA optimized by using additives and gels Optimalizovaná krystalizace flavoproteinu WrbA s využitím aditiv a gelů (cs) Crystallization of the flavoprotein WrbA optimized by using additives and gels (en)
skos:prefLabel	Crystallization of the flavoprotein WrbA optimized by using additives and gels Optimalizovaná krystalizace flavoproteinu WrbA s využitím aditiv a gelů (cs) Crystallization of the flavoprotein WrbA optimized by using additives and gels (en)
skos:notation	RIV/67179843:_____/05:00029273!RIV06-AV0-67179843
http://linked.open.../vavai/riv/strany	502;505
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ME 640), Z(AV0Z60870520), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	3-4
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Wolfová, Julie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	516602
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/05:00029273
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	crystalization; crystal (en)
http://linked.open.../riv/klicoveSlovo	crystalization crystal
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[E13E820B35F7]
http://linked.open...i/riv/nazevZdroje	Journal of Crystal Growth
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20640
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	284
http://linked.open...iv/tvurceVysledku	Wolfová, Julie
http://linked.open...n/vavai/riv/zamer	Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	0022-0248
number of pages	4 (xsd:int)

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