About: MicroRNA isolation and stability in stored RNA samples

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	MicroRNAs (miRNAs) are small RNA molecules, which act as post-transcriptional regulators of a gene expression, with important functions within the cell physiology. Whilst many authors have focused on the study of miRNA expression in physiological and pathological processes, various technical variables related to miRNA isolation have simultaneously emerged and the stability of the stored miRNA samples has been questioned. A robust method for RNA isolation is essential for reproducible results and miRNAs instability in the stored samples would make for an alarming situation for most expression studies. Here these issues are discussed and we investigate the stability of miRNAs isolated from clinical samples of B lymphocytes (chronic lymphocytic leukemia) by the most commonly utilized method based on a Trizol/TRI-Reagent solution (RNAs stored at -80 °C). MicroRNAs (miRNAs) are small RNA molecules, which act as post-transcriptional regulators of a gene expression, with important functions within the cell physiology. Whilst many authors have focused on the study of miRNA expression in physiological and pathological processes, various technical variables related to miRNA isolation have simultaneously emerged and the stability of the stored miRNA samples has been questioned. A robust method for RNA isolation is essential for reproducible results and miRNAs instability in the stored samples would make for an alarming situation for most expression studies. Here these issues are discussed and we investigate the stability of miRNAs isolated from clinical samples of B lymphocytes (chronic lymphocytic leukemia) by the most commonly utilized method based on a Trizol/TRI-Reagent solution (RNAs stored at -80 °C). (en)
Title	MicroRNA isolation and stability in stored RNA samples MicroRNA isolation and stability in stored RNA samples (en)
skos:prefLabel	MicroRNA isolation and stability in stored RNA samples MicroRNA isolation and stability in stored RNA samples (en)
skos:notation	RIV/65269705:_____/09:#0000788!RIV10-MZ0-65269705
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC06027), P(NR9293), S, Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Mráz, Marek Pospíšilová, Šárka Mayer, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Brno / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	326206
http://linked.open...ai/riv/idVysledku	RIV/65269705:_____/09:#0000788
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	miRNA isolation (en)
http://linked.open.../riv/klicoveSlovo	miRNA isolation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A70402274BFF]
http://linked.open...i/riv/nazevZdroje	Biochemical and biophysical research communications
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Application of MicroRNAs and Small Interfering RNAs in Diagnostics and Treatment of Leukemia University Research Centre - Czech Myeloma Group
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	390
http://linked.open...iv/tvurceVysledku	Mayer, Jiří Mráz, Marek Pospíšilová, Šárka
http://linked.open...ain/vavai/riv/wos	000271295500001
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	0006-291X
number of pages	5 (xsd:int)

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