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| Description
| - The present paper is focused on zinc(II) treatment effects on prostatic cell lines PC-3 (tumour) and PNT1A (non-tumour). Oxidative status of cells was monitored by evaluation of expression of metallothionein (MT) isoforms 1A and 2A at the mRNA and protein level, glutathione (oxidised and reduced), and intracellular zinc(II) after exposition to zinc(II) treatment at concentrations of 0-150 mu M using electrochemical methods, western blotting and fluorescent microscopy. A novel real-time impedance-based growth monitoring system was compared with widely used end-point MTT assay. Impedance-based IC50 for zinc(II) is 55.5 and 150.8 mu M for PC-3 and PNT1A, respectively. MTT-determined IC50 are } 1.3-fold higher. Impedance-based viability correlates with viable count (r } 0.92; p { 0.03), not with MTT. Two-fold lower intracellular zinc(II) in the tumour PC-3 cell line was found. After zinc(II) treatment } 2.6-fold increase of intracellular zinc(II) was observed in non-tumour PNT1A and in tumour PC-3 cells. In PC-3 cells, free and bound zinc(II) levels were enhanced more markedly as compared to PNT1A. PNT1A produced 4.2-fold less MT compared to PC3. PNT1A cells showed a 4.8-fold increase trend (r = 0.94; p = 0.005); PC-3 did show a significant trend at MT1 and MT2 protein levels (r = 0.93; p = 0.02) with nearly ten-fold increase after 100 mu M zinc(II) treatment.
- The present paper is focused on zinc(II) treatment effects on prostatic cell lines PC-3 (tumour) and PNT1A (non-tumour). Oxidative status of cells was monitored by evaluation of expression of metallothionein (MT) isoforms 1A and 2A at the mRNA and protein level, glutathione (oxidised and reduced), and intracellular zinc(II) after exposition to zinc(II) treatment at concentrations of 0-150 mu M using electrochemical methods, western blotting and fluorescent microscopy. A novel real-time impedance-based growth monitoring system was compared with widely used end-point MTT assay. Impedance-based IC50 for zinc(II) is 55.5 and 150.8 mu M for PC-3 and PNT1A, respectively. MTT-determined IC50 are } 1.3-fold higher. Impedance-based viability correlates with viable count (r } 0.92; p { 0.03), not with MTT. Two-fold lower intracellular zinc(II) in the tumour PC-3 cell line was found. After zinc(II) treatment } 2.6-fold increase of intracellular zinc(II) was observed in non-tumour PNT1A and in tumour PC-3 cells. In PC-3 cells, free and bound zinc(II) levels were enhanced more markedly as compared to PNT1A. PNT1A produced 4.2-fold less MT compared to PC3. PNT1A cells showed a 4.8-fold increase trend (r = 0.94; p = 0.005); PC-3 did show a significant trend at MT1 and MT2 protein levels (r = 0.93; p = 0.02) with nearly ten-fold increase after 100 mu M zinc(II) treatment. (en)
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| Title
| - Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining
- Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining (en)
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| skos:prefLabel
| - Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining
- Monitoring of the prostate tumour cells redox state and real-time proliferation by novel biophysical techniques and fluorescent staining (en)
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| skos:notation
| - RIV/62157124:16370/12:43871328!RIV13-MSM-16370___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ED1.1.00/02.0068), P(GAP102/11/1068), P(GP301/09/P436), S
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16370/12:43871328
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - glutathione; transporter; differentiation; metallothionein; zinc; acridine-orange; carcinoma-cells; liquid-chromatography; cancer cells; oxidative stress (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Babula, Petr
- Gumulec, Jaromír
- Kizek, René
- Křížková, Soňa
- Masařík, Michal
- Sochor, Jiří
- Sztalmachová, Markéta
- Zítka, Ondřej
- Cernei, Natalia
- Hlavna, Marian
- Raudenská, Martina
- Pavkova-Goldbergová, Monika
- Ruttkay-Nedecky, Branislav
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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