About: Square-wave voltammetry as a tool for investigation of doxorubicin interactions with DNA isolated from neuroblastoma cells

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About: Square-wave voltammetry as a tool for investigation of doxorubicin interactions with DNA isolated from neuroblastoma cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We investigated ethidium bromide intercalation into DNA molecule as a model system to test square-wave voltammetry (SWV) as a suitable method for this purpose We found that 0.13 μg EtBr intercalates into 1 μg dsDNA in average. Further, SWV was utilized for investigation of doxorubicin-DNA interactions. Intercalated doxorubicin reduced observed dsDNA cytosine and adenine (CA) signal, but also provided new signal called DOXO at - 0.35 V. This phenomenon was observed at both single and double stranded DNA standards. We also employed adsorptive transfer stripping technique coupled with SWV for study of doxorubicin-DNA interactions. Doxorubicin intercalation into dsDNA molecule adsorbed onto working electrode was fast, because we observed considerable changes in CA and DOXO signals after 360 s. Finally, we detected doxorubicin-DNA adducts formed in doxorubicin treated neuroblastoma cells. We investigated ethidium bromide intercalation into DNA molecule as a model system to test square-wave voltammetry (SWV) as a suitable method for this purpose We found that 0.13 μg EtBr intercalates into 1 μg dsDNA in average. Further, SWV was utilized for investigation of doxorubicin-DNA interactions. Intercalated doxorubicin reduced observed dsDNA cytosine and adenine (CA) signal, but also provided new signal called DOXO at - 0.35 V. This phenomenon was observed at both single and double stranded DNA standards. We also employed adsorptive transfer stripping technique coupled with SWV for study of doxorubicin-DNA interactions. Doxorubicin intercalation into dsDNA molecule adsorbed onto working electrode was fast, because we observed considerable changes in CA and DOXO signals after 360 s. Finally, we detected doxorubicin-DNA adducts formed in doxorubicin treated neuroblastoma cells. (en)
Title	Square-wave voltammetry as a tool for investigation of doxorubicin interactions with DNA isolated from neuroblastoma cells Square-wave voltammetry as a tool for investigation of doxorubicin interactions with DNA isolated from neuroblastoma cells (en)
skos:prefLabel	Square-wave voltammetry as a tool for investigation of doxorubicin interactions with DNA isolated from neuroblastoma cells Square-wave voltammetry as a tool for investigation of doxorubicin interactions with DNA isolated from neuroblastoma cells (en)
skos:notation	RIV/62157124:16370/09:00002084!RIV10-MSM-16370___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(IAA401990701), S, Z(MSM0021620813)
http://linked.open...iv/cisloPeriodika	3-5
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Babula, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Farmaceutická fakulta
http://linked.open...dnocenehoVysledku	343175
http://linked.open...ai/riv/idVysledku	RIV/62157124:16370/09:00002084
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Adsorptive transfer stripping technique; Doxorubicin; Intercalating drug; Nucleic acids; Voltammetry (en)
http://linked.open.../riv/klicoveSlovo	Doxorubicin Adsorptive transfer stripping technique Nucleic acids Intercalating drug Voltammetry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[164320DA9B98]
http://linked.open...i/riv/nazevZdroje	Electroanalysis
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of binding of platinum cytostatics to DNA structure; Influence of metallothionein
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	21
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Babula, Petr Eckschlager, Tomáš Kizek, Rene Stiborová, Marie Trnková, Libuše Huska, Dalibor Hrabeta, Jan
http://linked.open...ain/vavai/riv/wos	000264063500035
http://linked.open...n/vavai/riv/zamer	The Molecular Basis of Childhood Malignancies and Therapeutic Applications
issn	1040-0397
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	16370

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