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  • Protein analysis is very important both in terms of milk protein allergy, and of milk and dairy product adulteration (beta-lactoglobulin may be an important marker in the detection of milk adulteration). The aim of this study was to detect major whey proteins alfa-lactalbumin and beta-lactoglobulin and their genetic variants by reversed-phase high-performance liquid chromatography. Milk samples from cows (n = 40), goats (n = 40) and sheep (n = 40) were collected at two farms and milk bars in the Czech Republic from April to June 2010. The concentration of alfa-lactalbumin was higher in goat's milk (1.27 +- 0.05 g?l-1, P { 0.001) and cow's milk (1.16 +- 0.02 g?l-1, P = 0.0037) compared to sheep's milk (0.95 +- 0.06 g?l-1); however, concentration of alfa-lactalbumin in goat's milk and cow's milk did not differ significantly (P { 0.05). Goat's milk contained less beta-lactoglobulin (3.07 +- 0.08 g?l-1) compared to cow's milk (4.10 +- 0.04 g?l-1, P { 0.001) or sheep's milk (5.97 +- 0.24 g?l-1, P { 0.001). A highly significant positive correlation (r = 0.8686; P { 0.001) was found between fraction A and B of beta-lactoglobulin in sheep's milk, whereas in cow's milk there was a negative correlation (r = -0.3010; P = 0.0296). This study summarizes actual information of the whey protein content in different types
  • Protein analysis is very important both in terms of milk protein allergy, and of milk and dairy product adulteration (beta-lactoglobulin may be an important marker in the detection of milk adulteration). The aim of this study was to detect major whey proteins alfa-lactalbumin and beta-lactoglobulin and their genetic variants by reversed-phase high-performance liquid chromatography. Milk samples from cows (n = 40), goats (n = 40) and sheep (n = 40) were collected at two farms and milk bars in the Czech Republic from April to June 2010. The concentration of alfa-lactalbumin was higher in goat's milk (1.27 +- 0.05 g?l-1, P { 0.001) and cow's milk (1.16 +- 0.02 g?l-1, P = 0.0037) compared to sheep's milk (0.95 +- 0.06 g?l-1); however, concentration of alfa-lactalbumin in goat's milk and cow's milk did not differ significantly (P { 0.05). Goat's milk contained less beta-lactoglobulin (3.07 +- 0.08 g?l-1) compared to cow's milk (4.10 +- 0.04 g?l-1, P { 0.001) or sheep's milk (5.97 +- 0.24 g?l-1, P { 0.001). A highly significant positive correlation (r = 0.8686; P { 0.001) was found between fraction A and B of beta-lactoglobulin in sheep's milk, whereas in cow's milk there was a negative correlation (r = -0.3010; P = 0.0296). This study summarizes actual information of the whey protein content in different types (en)
Title
  • Determination of whey proteins in different types of milk
  • Determination of whey proteins in different types of milk (en)
skos:prefLabel
  • Determination of whey proteins in different types of milk
  • Determination of whey proteins in different types of milk (en)
skos:notation
  • RIV/62157124:16270/14:43872989!RIV15-MSM-16270___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S, Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 10863
http://linked.open...ai/riv/idVysledku
  • RIV/62157124:16270/14:43872989
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • RP-HPLC; sheep; goat; cow; beta-lactoglobulin; alfa-lactalbumin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [2BDD61D751C1]
http://linked.open...i/riv/nazevZdroje
  • Acta veterinaria Brno
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 83
http://linked.open...iv/tvurceVysledku
  • Bedáňová, Iveta
  • Borkovcová, Ivana
  • Ruprichová, Lenka
  • Vorlová, Lenka
  • Králová, Michaela
http://linked.open...ain/vavai/riv/wos
  • 000335204700012
http://linked.open...n/vavai/riv/zamer
issn
  • 0001-7213
number of pages
http://bibframe.org/vocab/doi
  • 10.2754/avb201483010067
http://localhost/t...ganizacniJednotka
  • 16270
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