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| Description
| - Lead(II) ions represent still threat to living organisms due to high burden of these ions in environment. The aim of this study was to design and perform experiments studying the interaction of lead(II) ions and proteins contained in egg albumen. For this purpose, we used electrochemical and electrophoretic techniques. We focused on a very detailed study of the interaction of lead(II) ions with the egg albumen proteins. Firstly, egg albumen was diluted with acetate buffer in the following ratios buffer: albumen - 1:0.5; 1: 1; 1: 2 and 1: 4. Additions of different concentrations of lead(II) ions into the diluted egg albumen were 0; 1; 5; 10; 25; 50; 100; 150; 200; 250; 500; 750 and 1,000 nM. Mixtures were then placed on thermoblock at 37 degrees C for 15, 30, 45 and 60 min. Based on determined protein profiles (as slopes of signal changes depending on the applied lead(II) concentration) at various dilutions it is clearly evident that the most significant changes are detected at ovotransferrin, avidin, lysozyme and ovomucoid. In addition, we employed optimized methods for studying of lead(II) induced complexes in died embryo of vulture. Contents of lead(II) ions, reduced and oxidised glutathione, metallothionein and zinc(II) ions were determined in albumen, yolk, liver, kidney, brain and bone obtained from the embryo.
- Lead(II) ions represent still threat to living organisms due to high burden of these ions in environment. The aim of this study was to design and perform experiments studying the interaction of lead(II) ions and proteins contained in egg albumen. For this purpose, we used electrochemical and electrophoretic techniques. We focused on a very detailed study of the interaction of lead(II) ions with the egg albumen proteins. Firstly, egg albumen was diluted with acetate buffer in the following ratios buffer: albumen - 1:0.5; 1: 1; 1: 2 and 1: 4. Additions of different concentrations of lead(II) ions into the diluted egg albumen were 0; 1; 5; 10; 25; 50; 100; 150; 200; 250; 500; 750 and 1,000 nM. Mixtures were then placed on thermoblock at 37 degrees C for 15, 30, 45 and 60 min. Based on determined protein profiles (as slopes of signal changes depending on the applied lead(II) concentration) at various dilutions it is clearly evident that the most significant changes are detected at ovotransferrin, avidin, lysozyme and ovomucoid. In addition, we employed optimized methods for studying of lead(II) induced complexes in died embryo of vulture. Contents of lead(II) ions, reduced and oxidised glutathione, metallothionein and zinc(II) ions were determined in albumen, yolk, liver, kidney, brain and bone obtained from the embryo. (en)
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| Title
| - Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods
- Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods (en)
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| skos:prefLabel
| - Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods
- Metallomics Study of Lead-Protein Interactions in Albumen by Electrochemical and Electrophoretic Methods (en)
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| skos:notation
| - RIV/62157124:16270/12:43871393!RIV13-MSM-16270___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ED1.1.00/02.0068), P(GA102/08/1546), V, Z(MSM0021622412)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16270/12:43871393
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - proteomics; spectrophotometry; metallothionein; capillary electrophoresis; heavy metal; differential pulse voltammetry; automated electrochemical detection; protein interaction (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - International journal of electrochemical science
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Beklová, Miroslava
- Hubálek, Jaromír
- Hynek, David
- Hájková, Pavlína
- Kizek, René
- Křížková, Soňa
- Krejčová, Ludmila
- Pikula, Jiří
- Pohanka, Miroslav
- Sochor, Jiří
- Trnková, Libuše
- Vrba, Radimír
- Ruttkay-Nedecký, Branislav
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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