About: Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk

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About: Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The occurrence of tetracycline resistance determinants in Escherichia coli recovered from raw cow's milk from the same farm sampled in two different time periods five years apart was investigated in this study. Detection of E. coli was performed and evaluated according to ISO 16649-2 and antibiotic resistance was screened by disk diffusion method. The polymerase chain reaction (PCR) was used to detect selected genes encoding resistance to tetracycline - tetA, tetB, tetC, tetD, and tetG. Of 89 samples of raw cow's milk, 84 (94.4%) were positive for E. coli. In total, 102 isolates were obtained. Fifty (49.0%) of these isolates were resistant to tetracycline. The most common gene detected in tetracycline-resistant isolates from 2010-2011was tetA (81.3 %), while tetB was most often (86.5%) found in isolates from 2005-2006. Neither the tetC, tetD, and tetG genes nor the co-occurrence of tetA and tetB genes were detected. None of the monitored genes was detected in two tetracycline-resistant isolates. Results of our study showed a significant shift in the distribution pattern of tetA and tetB genes in E. coli isolates from raw cow's milk five years apart. The occurrence of tetracycline resistance determinants in Escherichia coli recovered from raw cow's milk from the same farm sampled in two different time periods five years apart was investigated in this study. Detection of E. coli was performed and evaluated according to ISO 16649-2 and antibiotic resistance was screened by disk diffusion method. The polymerase chain reaction (PCR) was used to detect selected genes encoding resistance to tetracycline - tetA, tetB, tetC, tetD, and tetG. Of 89 samples of raw cow's milk, 84 (94.4%) were positive for E. coli. In total, 102 isolates were obtained. Fifty (49.0%) of these isolates were resistant to tetracycline. The most common gene detected in tetracycline-resistant isolates from 2010-2011was tetA (81.3 %), while tetB was most often (86.5%) found in isolates from 2005-2006. Neither the tetC, tetD, and tetG genes nor the co-occurrence of tetA and tetB genes were detected. None of the monitored genes was detected in two tetracycline-resistant isolates. Results of our study showed a significant shift in the distribution pattern of tetA and tetB genes in E. coli isolates from raw cow's milk five years apart. (en)
Title	Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk (en)
skos:prefLabel	Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk (en)
skos:notation	RIV/62157124:16270/12:43871277!RIV13-MSM-16270___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika	February
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Janštová, Bohumíra Karpíšková, Renata Cupáková, Šárka Skočková, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinární hygieny a ekologie
http://linked.open...dnocenehoVysledku	130471
http://linked.open...ai/riv/idVysledku	RIV/62157124:16270/12:43871277
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Escherichia coli; tet-genes; tetracycline; Resistance (en)
http://linked.open.../riv/klicoveSlovo	Resistance tet-genes tetracycline Escherichia coli
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[DE0C86A046D5]
http://linked.open...i/riv/nazevZdroje	Journal of Microbiology, Biotechnology and Food Sciences
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	1
http://linked.open...iv/tvurceVysledku	Janštová, Bohumíra Karpíšková, Renata Cupáková, Šárka Skočková, Alena
http://linked.open...n/vavai/riv/zamer	Veterinary aspects of food safety and quality
issn	1338-5178
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	16270

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