About: Comparison of the Results of the ELISA, Histochemical, and Immunohistochemical Detection of Soya Proteins in Meat Products

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This work compares the commonly used immunochemical methods for soya protein detection and alternative microscopic methods. Immunochemical methods were represented by the competitive ELISA method. Histochemical and immunohistochemical methods were used for microscopical examination. From a group of 252 meat products, each sample was examined for soya proteins by ELISA, histochemical, and immunohistochemical methods. The products came from the following categories: cooked sausages, ham, dry cooked sausages, and fermented sausages. The results showed that the highest accuracy was achieved by immunohistochemical examination. However, in the category of cooked sausages, this result was not statistically significant. Since the results in the individual categories differed, our results demonstrate that one single method does not always provide reliable and completely objective results. Immunohistochemical methods seem to be the most suitable for the verification of the reference immunochemical method results and prevention of false results. This work compares the commonly used immunochemical methods for soya protein detection and alternative microscopic methods. Immunochemical methods were represented by the competitive ELISA method. Histochemical and immunohistochemical methods were used for microscopical examination. From a group of 252 meat products, each sample was examined for soya proteins by ELISA, histochemical, and immunohistochemical methods. The products came from the following categories: cooked sausages, ham, dry cooked sausages, and fermented sausages. The results showed that the highest accuracy was achieved by immunohistochemical examination. However, in the category of cooked sausages, this result was not statistically significant. Since the results in the individual categories differed, our results demonstrate that one single method does not always provide reliable and completely objective results. Immunohistochemical methods seem to be the most suitable for the verification of the reference immunochemical method results and prevention of false results. (en)
Title	Comparison of the Results of the ELISA, Histochemical, and Immunohistochemical Detection of Soya Proteins in Meat Products Comparison of the Results of the ELISA, Histochemical, and Immunohistochemical Detection of Soya Proteins in Meat Products (en)
skos:prefLabel	Comparison of the Results of the ELISA, Histochemical, and Immunohistochemical Detection of Soya Proteins in Meat Products Comparison of the Results of the ELISA, Histochemical, and Immunohistochemical Detection of Soya Proteins in Meat Products (en)
skos:notation	RIV/62157124:16270/11:43870637!RIV12-MZE-16270___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1B53004), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Řezáčová Lukášková, Zuzana Tremlová, Bohuslava Pokorná, Jana Pospiech, Matej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinární hygieny a ekologie
http://linked.open...dnocenehoVysledku	191279
http://linked.open...ai/riv/idVysledku	RIV/62157124:16270/11:43870637
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	histochemistry; immunochemistry; allergen; ELISA (en)
http://linked.open.../riv/klicoveSlovo	ELISA allergen histochemistry immunochemistry
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[C836ACFF5735]
http://linked.open...i/riv/nazevZdroje	Czech Journal of Food Sciences
http://linked.open...in/vavai/riv/obor	GM
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Qualitative and quantitative diagnostics of particles of plant and animal origin in foodstuffs using histochemical or immunohistochemical methods.
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Eva, Renčová Pokorná, Jana Pospiech, Matej Randulová, Zdeňka Tremlová, Bohuslava Řezáčová Lukášková, Zuzana
http://linked.open...ain/vavai/riv/wos	000296874400002
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	1212-1800
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	16270

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