About: Analysis of DNA for Differentiation of Fish Species in Seafood Samples

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify fish species and to perform their authentication in commercial seafood products. The mitochondrial cytochrome b gene provides very low intraspecies variability, thus allowing the unequivocal identification of fish species. Universal primers were used for PCR amplification of 464-bp long fragments of this gene. The PCR products were digested with restriction enzymes AluI, HinfI, HaeIII, DdeI, NlaIII, HincII, MboII. Sixty fish samples were obtained from the local markets in the Czech Republic. At 47 samples (78.3%) the results were in agreement with declarations of the producers and 10 samples (16.7%) contained other fish species. There were not great differences between fresh fillets (chilled, frozen etc.) and heat processed foodstuffs (tinned, smoked, etc.). Correct designation was at 72.3% and 81.6% samples, respectively. Even if in 3 cases the analysis was unsuccessful the method is usefull Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify fish species and to perform their authentication in commercial seafood products. The mitochondrial cytochrome b gene provides very low intraspecies variability, thus allowing the unequivocal identification of fish species. Universal primers were used for PCR amplification of 464-bp long fragments of this gene. The PCR products were digested with restriction enzymes AluI, HinfI, HaeIII, DdeI, NlaIII, HincII, MboII. Sixty fish samples were obtained from the local markets in the Czech Republic. At 47 samples (78.3%) the results were in agreement with declarations of the producers and 10 samples (16.7%) contained other fish species. There were not great differences between fresh fillets (chilled, frozen etc.) and heat processed foodstuffs (tinned, smoked, etc.). Correct designation was at 72.3% and 81.6% samples, respectively. Even if in 3 cases the analysis was unsuccessful the method is usefull (en)
Title	Analysis of DNA for Differentiation of Fish Species in Seafood Samples Analysis of DNA for Differentiation of Fish Species in Seafood Samples (en)
skos:prefLabel	Analysis of DNA for Differentiation of Fish Species in Seafood Samples Analysis of DNA for Differentiation of Fish Species in Seafood Samples (en)
skos:notation	RIV/62157124:16270/10:00002189!RIV10-MSM-16270___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kasalová, Janka Bořilová, Gabriela Nebola, Mojmír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinární hygieny a ekologie
http://linked.open...dnocenehoVysledku	246378
http://linked.open...ai/riv/idVysledku	RIV/62157124:16270/10:00002189
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fish species; PCR-RFLP; seafood products (en)
http://linked.open.../riv/klicoveSlovo	PCR-RFLP seafood products fish species
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[BFC122B8BC3B]
http://linked.open...i/riv/nazevZdroje	Bulletin of the veterinary institute in Pulawy
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Bořilová, Gabriela Nebola, Mojmír Kasalová, Janka
http://linked.open...n/vavai/riv/zamer	Veterinary aspects of food safety and quality
issn	0042-4870
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	16270

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