About: Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica).

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About: Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica). Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Metallothioneins (MT) play a key role in maintaining the homeostasis of essential metals and in protecting of cells against metal toxicity as well as cell oxidative damaging. The aim of this work is to propose a new approach for processing a biological sample for analysis of thiols including metallothioneins. Moreover, the proposed procedure is tested on quantification of MT and total thiol content in blood serum of pig (Sus scrofa domestica), which has not previously been performed. The blood serum (10 ml) was collected and transferred to 0.2 M phosphate buffer (990 ml). The sample (100 x diluted) was placed in a thermomixer, where heat denaturation of most of the proteins proceeded. The processed blood serum sample was electrochemically measured to determine total content of thiols (cysteine, glutathione, metallothionein and other low molecular thermostable thiols) and content of MT. The average level of the thiols and MT were estimated as 165 +-20 mM and 5.2 +- 0.6 mM, respectively. Metallothioneins (MT) play a key role in maintaining the homeostasis of essential metals and in protecting of cells against metal toxicity as well as cell oxidative damaging. The aim of this work is to propose a new approach for processing a biological sample for analysis of thiols including metallothioneins. Moreover, the proposed procedure is tested on quantification of MT and total thiol content in blood serum of pig (Sus scrofa domestica), which has not previously been performed. The blood serum (10 ml) was collected and transferred to 0.2 M phosphate buffer (990 ml). The sample (100 x diluted) was placed in a thermomixer, where heat denaturation of most of the proteins proceeded. The processed blood serum sample was electrochemically measured to determine total content of thiols (cysteine, glutathione, metallothionein and other low molecular thermostable thiols) and content of MT. The average level of the thiols and MT were estimated as 165 +-20 mM and 5.2 +- 0.6 mM, respectively. (en)
Title	Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica). Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica). (en)
skos:prefLabel	Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica). Employing voltammetry for determination of low molecular mass thiols and metallothionein in blood of pig (Sus scrofa domestica). (en)
skos:notation	RIV/62157124:16270/09:00002134!RIV10-MSM-16270___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pikula, Jiří Beklová, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinární hygieny a ekologie
http://linked.open...dnocenehoVysledku	313125
http://linked.open...ai/riv/idVysledku	RIV/62157124:16270/09:00002134
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	electrochemical method; differential pulse voltammetry; Brdicka reaction; protein; pig (en)
http://linked.open.../riv/klicoveSlovo	electrochemical method protein pig differential pulse voltammetry Brdicka reaction
http://linked.open...odStatuVydavatele	HR - Chorvatská republika
http://linked.open...ontrolniKodProRIV	[F1227FEA43E2]
http://linked.open...i/riv/nazevZdroje	Natura Croatica
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Doležal, Petr Kizek, René Mareš, Pavel Pikula, Jiří Trnková, Libuše Zeman, Ladislav
http://linked.open...n/vavai/riv/zamer	Veterinary aspects of food safety and quality
issn	1330-0520
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	16270

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