About: Electrochemical determination of metallothionein in the domestic fowl

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Metallothionein (MT) belongs to group of intracellular, low-molecular and cysteine-rich proteins with a molecular weight from 6 to 10 kDa. Owing to their high affinity to heavy metals (Zn, Cd, As, etc.) their main role is homeostatic control and detoxification of metal ions in an organism. In the present work we aimed at suggesting and utilizing electroanalytical techniques to determine content of MT in the blood serum of domestic fowls. Electrochemical measurements were performed with an AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. Particularly, MT was detected by adsorptive transfer stripping technique in connection with differential pulse voltammetry. The detection limit of MT was estimated down to 100 fM (standards only) or down to 100 pM measured in the presence of blood serum. The average content of MT was 21.3 μM. The MT level in hens was about 25 % higher than in cocks. This phenomenon can be related to higher demands on the content of this prot Metallothionein (MT) belongs to group of intracellular, low-molecular and cysteine-rich proteins with a molecular weight from 6 to 10 kDa. Owing to their high affinity to heavy metals (Zn, Cd, As, etc.) their main role is homeostatic control and detoxification of metal ions in an organism. In the present work we aimed at suggesting and utilizing electroanalytical techniques to determine content of MT in the blood serum of domestic fowls. Electrochemical measurements were performed with an AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. Particularly, MT was detected by adsorptive transfer stripping technique in connection with differential pulse voltammetry. The detection limit of MT was estimated down to 100 fM (standards only) or down to 100 pM measured in the presence of blood serum. The average content of MT was 21.3 μM. The MT level in hens was about 25 % higher than in cocks. This phenomenon can be related to higher demands on the content of this prot (en)
Title	Electrochemical determination of metallothionein in the domestic fowl Electrochemical determination of metallothionein in the domestic fowl (en)
skos:prefLabel	Electrochemical determination of metallothionein in the domestic fowl Electrochemical determination of metallothionein in the domestic fowl (en)
skos:notation	RIV/62157124:16270/09:00002133!RIV10-MSM-16270___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6215712402)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pikula, Jiří Beklová, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinární hygieny a ekologie
http://linked.open...dnocenehoVysledku	312805
http://linked.open...ai/riv/idVysledku	RIV/62157124:16270/09:00002133
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	electrochemical method; differential pulse voltammetry; Brdicka reaction; protein; bird (en)
http://linked.open.../riv/klicoveSlovo	electrochemical method protein bird differential pulse voltammetry Brdicka reaction
http://linked.open...odStatuVydavatele	HR - Chorvatská republika
http://linked.open...ontrolniKodProRIV	[0FED58BB50F9]
http://linked.open...i/riv/nazevZdroje	Natura Croatica
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	17
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Beklová, Miroslava Kizek, René Pikula, Jiří Šobrová, Pavlína Fabrik, Ivan
http://linked.open...n/vavai/riv/zamer	Veterinary aspects of food safety and quality
issn	1330-0520
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	16270

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