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rdf:type
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Description
| - Proteomika, studium proteomu, zahrnuje identifikaci, charakteristiku a kvantifikaci kompletních směsí proteinů, které exprimuje celý genom v průběhu života dané buňky, tkáně nebo organismu včetně izoforem, polymorfismu a různých modifikací, protein-proteinových interakcí a strukturálního popisu proteinů a jejich komplexů. Většina biologických funkcí je vázána na proteiny a proto, abychom pochopili, jak buňky fungují, musíme studovat proteiny v buňkách, jejich funkci, jejich interakce a množství v analyzovaném vzorku. V této studii jsme stanovovali různé peptidy a proteiny (cystein, oxidovaný a redukovaný glutation, fytochelatiny, metalotionein a bovinní sérový albumin) pomocí metody %22adsorptive transfer stripping voltametry (AdTS) a %22chronopotentiometric stripping analysis (CPSA). Byla provedena optimalizace parametrů těchto technik a následně bylo možno stanovovat řádově stovky zeptomolů daných sloučenin. (cs)
- Proteomics, the study of the proteome, has come to encompass the identification, characterisation and quantification of the complete set of proteins expressed by the entire genome in the lifetime of a given cell, tissue or organism, including isoforms, polymorphisms and modifications, protein-protein interactions and the structural description of proteins and their complexes. Most biological functions are carried out by proteins, and to understand how cells work, one must study the proteins present, what they do, how they interact with one another and how many of them are in an analysed sample. Here, we attempted to determine different peptides and proteins (cysteine, oxidized and reduced glutathione, phytochelatins, metallothionein and bovine serum albumin) by adsorptive transfer stripping voltammetry (AdTS) chronopotentiometric stripping analysis (CPSA). We optimized the parameters of the technique and, consequently, were able to determine from units to hundreds zeptomoles of the compounds of intere
- Proteomics, the study of the proteome, has come to encompass the identification, characterisation and quantification of the complete set of proteins expressed by the entire genome in the lifetime of a given cell, tissue or organism, including isoforms, polymorphisms and modifications, protein-protein interactions and the structural description of proteins and their complexes. Most biological functions are carried out by proteins, and to understand how cells work, one must study the proteins present, what they do, how they interact with one another and how many of them are in an analysed sample. Here, we attempted to determine different peptides and proteins (cysteine, oxidized and reduced glutathione, phytochelatins, metallothionein and bovine serum albumin) by adsorptive transfer stripping voltammetry (AdTS) chronopotentiometric stripping analysis (CPSA). We optimized the parameters of the technique and, consequently, were able to determine from units to hundreds zeptomoles of the compounds of intere (en)
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Title
| - Nový nástroj pro určování proteinů: chronopotenciometrická analýza (cs)
- A new tool for determination of proteins: chronopotentiometric stripping analysis
- A new tool for determination of proteins: chronopotentiometric stripping analysis (en)
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skos:prefLabel
| - Nový nástroj pro určování proteinů: chronopotenciometrická analýza (cs)
- A new tool for determination of proteins: chronopotentiometric stripping analysis
- A new tool for determination of proteins: chronopotentiometric stripping analysis (en)
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skos:notation
| - RIV/62157124:16270/06:00000987!RIV07-MSM-16270___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16270/06:00000987
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Proteomics; proteome (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/statKonaniAkce
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http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Beklová, Miroslava
- Havel, Ladislav
- Kizek, René
- Pikula, Jiří
- Svoboda, Michal
- Trnková, Libuše
- Petrlová, Jitka
- Wang, Joseph
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/ukonceniAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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http://localhost/t...ganizacniJednotka
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