| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Objective: Dogs with chronic renal failure (CRF) may suffer from extreme azotemia. Urea enters erythrocytes (RBC) through its membrane. Once there, it destabilize proteins and change the set point of volume of RBC to lower values. The RBC with smaller MCV is more resistant to hypotonic environment. Previous studies on osmotic fragility revealed contrarily results. The aim of this study was to find an effect of azotaemia caused by chronic renal failure on RBC in dogs. Methods: Thirteen dogs with CRF and eighteen healthy dogs were enrolled in this study. Twenty-five microliters of heparin blood was mixed with 2,5ml of NaCl solution with decreasing concentration 0,9% - 0,1%. After incubation of 30 minutes and centrifugation the absorbance of the supernatant was measured by ELISA reader at wavelength of 540 nm. The cumulative curve was made. Median, minimal and maximal erythrocyte osmotic fragility (OF) was examined from the curve as a concentration of NaCl at which 50%, 5% and 90% of the erythrocytes were hemolyzed. Differences between groups were evaluated by Mann-Whitney U test, for correlation analysis Spearman test was used. Results: OF in patients and in control group was not significantly different: minimal OF (p=0,75), median OF (p=0,6) and maximal OF (p=0,96), but patients with urea concentration over 60mmol/l had median OF higher than control dogs (p=0,07). Conclusion: OF of RBC seems to be not affected by azotaemia in dogs with chronic renal failure.
- Objective: Dogs with chronic renal failure (CRF) may suffer from extreme azotemia. Urea enters erythrocytes (RBC) through its membrane. Once there, it destabilize proteins and change the set point of volume of RBC to lower values. The RBC with smaller MCV is more resistant to hypotonic environment. Previous studies on osmotic fragility revealed contrarily results. The aim of this study was to find an effect of azotaemia caused by chronic renal failure on RBC in dogs. Methods: Thirteen dogs with CRF and eighteen healthy dogs were enrolled in this study. Twenty-five microliters of heparin blood was mixed with 2,5ml of NaCl solution with decreasing concentration 0,9% - 0,1%. After incubation of 30 minutes and centrifugation the absorbance of the supernatant was measured by ELISA reader at wavelength of 540 nm. The cumulative curve was made. Median, minimal and maximal erythrocyte osmotic fragility (OF) was examined from the curve as a concentration of NaCl at which 50%, 5% and 90% of the erythrocytes were hemolyzed. Differences between groups were evaluated by Mann-Whitney U test, for correlation analysis Spearman test was used. Results: OF in patients and in control group was not significantly different: minimal OF (p=0,75), median OF (p=0,6) and maximal OF (p=0,96), but patients with urea concentration over 60mmol/l had median OF higher than control dogs (p=0,07). Conclusion: OF of RBC seems to be not affected by azotaemia in dogs with chronic renal failure. (en)
|
| Title
| - The effect of azotemia on erythrocyte osmotic fragility in dogs with chronic and end-stage renal failure.
- The effect of azotemia on erythrocyte osmotic fragility in dogs with chronic and end-stage renal failure. (en)
|
| skos:prefLabel
| - The effect of azotemia on erythrocyte osmotic fragility in dogs with chronic and end-stage renal failure.
- The effect of azotemia on erythrocyte osmotic fragility in dogs with chronic and end-stage renal failure. (en)
|
| skos:notation
| - RIV/62157124:16170/14:43873303!RIV15-MSM-16170___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16170/14:43873303
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - chronic renal failure; osmotic resistance (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Uhríková, Ivana
- Doubek, Jaroslav
- Salášková, Pavla
|
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)