About: Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov     Goto   Sponge   NotDistinct   Permalink

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Description
  • A novel bacterial strain, designated as M8T, was isolated from milk of macaque female bred in captivity. The strain was Gram-positive, anaerobic, irregular coccoid rod without catalase activity. Analysis of 16S rRNA gene sequence similarity revealed that the isolate was most closely related to Alloscardovia omnicolens CCUG 31649T (96.4 %) and Metascardovia criceti OMB 105T (96.6%). Sequences of hsp60, fusA, and xfp genes also confirmed that the strains is most closely related to the type strains of A. omnicolens and M. criceti. The isolate produced fructose-6-phosphate phosphoketolase what is in agreement with the classification within the family Bifidobacteriaceae. The major fatty acids were C18:1 omega 9c (35.8 %), C16:1 (6.2 %) and C14:0 (5.7 %). Polar lipid analysis revealed five different glycolipids, two unidentified phospholipids and diphosphatidylglycerol. The peptidoglycan was of the type A4alpha L-Lys-D-Asp with the presence of D(L)-alanine, D-glutamine, D-asparagine and L-lysine. The DNA G+C content of the strain M8T was 50.1 mol%. On the basis of genetic, phylogenetic and phenotypic data, the identity of Alloscardovia macacae sp. nov. (M8T=DSM 24762T = CCM 7944T) is confirmed. In addition, our results also revealed that Alloscardovia omnicolens DSM 21503T and Metascardovia criceti DSM 17774T do not belong to different genera within the family Bifidobacteriaceae. We therefore propose to reclassify Metascardovia criceti as Alloscardovia criceti comb. nov.
  • A novel bacterial strain, designated as M8T, was isolated from milk of macaque female bred in captivity. The strain was Gram-positive, anaerobic, irregular coccoid rod without catalase activity. Analysis of 16S rRNA gene sequence similarity revealed that the isolate was most closely related to Alloscardovia omnicolens CCUG 31649T (96.4 %) and Metascardovia criceti OMB 105T (96.6%). Sequences of hsp60, fusA, and xfp genes also confirmed that the strains is most closely related to the type strains of A. omnicolens and M. criceti. The isolate produced fructose-6-phosphate phosphoketolase what is in agreement with the classification within the family Bifidobacteriaceae. The major fatty acids were C18:1 omega 9c (35.8 %), C16:1 (6.2 %) and C14:0 (5.7 %). Polar lipid analysis revealed five different glycolipids, two unidentified phospholipids and diphosphatidylglycerol. The peptidoglycan was of the type A4alpha L-Lys-D-Asp with the presence of D(L)-alanine, D-glutamine, D-asparagine and L-lysine. The DNA G+C content of the strain M8T was 50.1 mol%. On the basis of genetic, phylogenetic and phenotypic data, the identity of Alloscardovia macacae sp. nov. (M8T=DSM 24762T = CCM 7944T) is confirmed. In addition, our results also revealed that Alloscardovia omnicolens DSM 21503T and Metascardovia criceti DSM 17774T do not belong to different genera within the family Bifidobacteriaceae. We therefore propose to reclassify Metascardovia criceti as Alloscardovia criceti comb. nov. (en)
Title
  • Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov
  • Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov (en)
skos:prefLabel
  • Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov
  • Alloscardovia macacae sp. nov., isolated from the milk of macaque (Macaca mulatta), emended description of the genus Alloscardovia and proposal of Alloscardovia criceti comb. nov (en)
skos:notation
  • RIV/62157124:16170/13:43872051!RIV14-MSM-16170___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • V
http://linked.open...iv/cisloPeriodika
  • December
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 60130
http://linked.open...ai/riv/idVysledku
  • RIV/62157124:16170/13:43872051
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • multilocus sequence analysis; Bifidobacteriaceae; macaque; Alloscardovia (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [C2409EC81397]
http://linked.open...i/riv/nazevZdroje
  • International Journal of Systematic and Evolutionary Microbiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 63
http://linked.open...iv/tvurceVysledku
  • Benada, Oldřich
  • Killer, Jiří
  • Kopečný, Jan
  • Profousová, Ilona
  • Vlková, Eva
  • Havlík, Jaroslav
  • Kofroňová, Olga
  • Rada, Vojtěch
  • Ročková, Šárka
  • Bunešová, Věra
  • Pechar, Radko
http://linked.open...ain/vavai/riv/wos
  • 000330922400011
issn
  • 1466-5026
number of pages
http://bibframe.org/vocab/doi
  • 10.1099/ijs.0.051326-0
http://localhost/t...ganizacniJednotka
  • 16170
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