About: InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus     Goto   Sponge   NotDistinct   Permalink

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  • Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch (TM) TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA). is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24 h. In this study we successfully cultivated P. hominis in InPouch (TM) TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch (TM) TF-Feline medium. PCR-based verification of culture-positive samples prior the potentially neurotoxic ronidazole treatment is recommended.
  • Trichomonads are a group of anaerobic flagellates. Two species of intestinal trichomonads have been described in cats - pathogenic Tritrichomonas foetus and probably harmless Pentatrichomonas hominis. InPouch (TM) TF-Feline medium (Bio-Med Diagnostics, White City, Oregon, USA). is considered the gold standard for diagnosis of T. foetus infection in cats. It is commercially available, relatively cheap and easy-to-use. The medium is intended to be highly specific to T. foetus as morphologically similar Pentatrichomonas hominis and Giardia sp. do not survive here longer than 24 h. In this study we successfully cultivated P. hominis in InPouch (TM) TF-Feline medium for 3 days after inoculation with cat faeces. The identity of the organism was assessed by sequencing of SSU rDNA and ITS region. Possible coinfection with T. foetus was ruled out using Tritrichomonas-specific PCR. Our results suggest possible misdiagnosis of tritrichomonosis in cats using InPouch (TM) TF-Feline medium. PCR-based verification of culture-positive samples prior the potentially neurotoxic ronidazole treatment is recommended. (en)
Title
  • InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus
  • InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus (en)
skos:prefLabel
  • InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus
  • InPouch (TM) TF-Feline medium is not specific for Tritrichomonas foetus (en)
skos:notation
  • RIV/62157124:16170/13:43871792!RIV14-MSM-16170___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, N
http://linked.open...iv/cisloPeriodika
  • 3-4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 80383
http://linked.open...ai/riv/idVysledku
  • RIV/62157124:16170/13:43871792
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Feline trichomonosis; Long-term large bowel diarrhoea; InPouch (TM) TF-Feline; Pentatrichomonas hominis; Tritrichomonas foetus (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [9F128B670E56]
http://linked.open...i/riv/nazevZdroje
  • Veterinary Parasitology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 196
http://linked.open...iv/tvurceVysledku
  • Husník, Roman
  • Svoboda, Miroslav
  • Svobodová, Vlasta
  • Čepička, Ivan
  • Horáčková, Kateřina
  • Ceplecha, Václav
http://linked.open...ain/vavai/riv/wos
  • 000323864500036
issn
  • 0304-4017
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.vetpar.2013.04.015
http://localhost/t...ganizacniJednotka
  • 16170
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