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Description
| - Caspase-3 is an executive caspase, in the central position within apoptotic machinery. Investigation of this molecules is of a great importance for basic as well as applied research. In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. Three different instrumental arrangements were compared for the determination of caspase-3 in cells. The best setting of the miniaturized system detected the mean amount as low as about 6.5 fg corresponding to 122000 molecules of caspase-3 in individual apoptotic cells.
- Caspase-3 is an executive caspase, in the central position within apoptotic machinery. Investigation of this molecules is of a great importance for basic as well as applied research. In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. Three different instrumental arrangements were compared for the determination of caspase-3 in cells. The best setting of the miniaturized system detected the mean amount as low as about 6.5 fg corresponding to 122000 molecules of caspase-3 in individual apoptotic cells. (en)
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Title
| - Bioluminescence determination of active caspase-3 in single apoptotic cells
- Bioluminescence determination of active caspase-3 in single apoptotic cells (en)
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skos:prefLabel
| - Bioluminescence determination of active caspase-3 in single apoptotic cells
- Bioluminescence determination of active caspase-3 in single apoptotic cells (en)
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skos:notation
| - RIV/62157124:16170/13:43871765!RIV14-GA0-16170___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - I, P(GAP206/11/2377), P(GAP502/12/1285)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16170/13:43871765
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - bioluminiscence; caspase 3 (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Foret, František
- Klepárník, Karel
- Lišková, Marcela
- Přikryl, Jan
- Matalová, Eva
- Hegrová, Jitka
- Švandová, Eva
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
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http://localhost/t...ganizacniJednotka
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