About: Bioluminescence determination of active caspase-3 in single apoptotic cells

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Caspase-3 is an executive caspase, in the central position within apoptotic machinery. Investigation of this molecules is of a great importance for basic as well as applied research. In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. Three different instrumental arrangements were compared for the determination of caspase-3 in cells. The best setting of the miniaturized system detected the mean amount as low as about 6.5 fg corresponding to 122000 molecules of caspase-3 in individual apoptotic cells. Caspase-3 is an executive caspase, in the central position within apoptotic machinery. Investigation of this molecules is of a great importance for basic as well as applied research. In this work we report on the development of a high-sensitivity instrumentation and protocol for detection of active caspase-3 in individual mammalian apoptotic cells. Three different instrumental arrangements were compared for the determination of caspase-3 in cells. The best setting of the miniaturized system detected the mean amount as low as about 6.5 fg corresponding to 122000 molecules of caspase-3 in individual apoptotic cells. (en)
Title	Bioluminescence determination of active caspase-3 in single apoptotic cells Bioluminescence determination of active caspase-3 in single apoptotic cells (en)
skos:prefLabel	Bioluminescence determination of active caspase-3 in single apoptotic cells Bioluminescence determination of active caspase-3 in single apoptotic cells (en)
skos:notation	RIV/62157124:16170/13:43871765!RIV14-GA0-16170___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP206/11/2377), P(GAP502/12/1285)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Matalová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinárního lékařství
http://linked.open...dnocenehoVysledku	63488
http://linked.open...ai/riv/idVysledku	RIV/62157124:16170/13:43871765
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bioluminiscence; caspase 3 (en)
http://linked.open.../riv/klicoveSlovo	caspase 3 bioluminiscence
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[D92FCBA0CA0E]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Single-cell analysis by mass spectrometry Apoptotic and non-apoptotic functions of caspases in hard tissue formation
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Foret, František Klepárník, Karel Lišková, Marcela Přikryl, Jan Matalová, Eva Hegrová, Jitka Švandová, Eva
http://linked.open...ain/vavai/riv/wos	000320385400009
issn	0173-0835
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/elps.201200675
http://localhost/t...ganizacniJednotka	16170

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