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| rdf:type
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| Description
| - The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%, p { 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7-9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%, p { 0.05) in experiment 1. The fluid from ovarian cysts } 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO(3), BE, pCO(2) and pO(2) (experiment 2). The indicators were compared between samples 1 and 2. Higher pO(2) as well as pH and lower pCO(2) in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration.
- The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%, p { 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7-9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%, p { 0.05) in experiment 1. The fluid from ovarian cysts } 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO(3), BE, pCO(2) and pO(2) (experiment 2). The indicators were compared between samples 1 and 2. Higher pO(2) as well as pH and lower pCO(2) in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration. (en)
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| Title
| - Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle
- Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle (en)
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| skos:prefLabel
| - Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle
- Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle (en)
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| skos:notation
| - RIV/62157124:16170/11:43870732!RIV12-MSM-16170___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16170/11:43870732
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - acid-base indicators; cysts; follicles; ovum pick-up; collection of follicular fluid; heifer; Cow (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Doležel, Radovan
- Malá, Jana
- Pechová, Alena
- Čech, Svatopluk
- Indrová, Eva
- Lopatářová, Miloslava
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
| |
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