About: Conception rate after sex determination and cryopreservation of D7 bovine embryos

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We evaluated conception rate after sex determination and subsequent freezing of D 7 bovine embryos and compared it with transfer of fresh female embryos. Fresh embryos were blade biopsied (n=172) and then transferred. High quality embryos (n=112) were biopsied by cell aspiration and cryopreserved. Sex determination (PCR using primers for the Y-chromosome determinant) was successfully completed in 91.3% of fresh (44% female) and in 87.5% of freeze-thawed embryos (45.9% female). The pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. Pregnancy rate after transfer of freeze-thawed sexed (female) embryos was 48.8%. A similar conception rate was found in intact embryos (50.7%, control 2). The results demonstrate that these techniques allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in ET programs. We evaluated conception rate after sex determination and subsequent freezing of D 7 bovine embryos and compared it with transfer of fresh female embryos. Fresh embryos were blade biopsied (n=172) and then transferred. High quality embryos (n=112) were biopsied by cell aspiration and cryopreserved. Sex determination (PCR using primers for the Y-chromosome determinant) was successfully completed in 91.3% of fresh (44% female) and in 87.5% of freeze-thawed embryos (45.9% female). The pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. Pregnancy rate after transfer of freeze-thawed sexed (female) embryos was 48.8%. A similar conception rate was found in intact embryos (50.7%, control 2). The results demonstrate that these techniques allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in ET programs. (en)
Title	Conception rate after sex determination and cryopreservation of D7 bovine embryos Conception rate after sex determination and cryopreservation of D7 bovine embryos (en)
skos:prefLabel	Conception rate after sex determination and cryopreservation of D7 bovine embryos Conception rate after sex determination and cryopreservation of D7 bovine embryos (en)
skos:notation	RIV/62157124:16170/10:00003003!RIV11-MSM-16170___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6215712403)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Doležel, Radovan Čech, Svatopluk Ševelová, Helena Lopatářová, Miloslava Holý, Luboš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinárního lékařství
http://linked.open...dnocenehoVysledku	251780
http://linked.open...ai/riv/idVysledku	RIV/62157124:16170/10:00003003
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cattle; superovulation; sexing; polymerase chain reaction; cryopreservation; embryo transfer (en)
http://linked.open.../riv/klicoveSlovo	polymerase chain reaction superovulation cryopreservation cattle embryo transfer sexing
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[8BD980B58EA8]
http://linked.open...i/riv/nazevZdroje	Veterinární medicína
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Doležel, Radovan Čech, Svatopluk Lopatářová, Miloslava Holý, Luboš Lálová, Helena Krontorád, Petr
http://linked.open...ain/vavai/riv/wos	000275478200002
http://linked.open...n/vavai/riv/zamer	Active creation of health, yield and performance of the large animals
issn	0375-8427
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	16170

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