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Description
  • Produkce reaktivních forem dusíku v průběhu ontogeneze klesá a je přechodně zvýšená v době odstavu. (cs)
  • The aim of this work was to evaluate ontogeny of reactive nitrogen species (RNS) production by peripheral blood phagocytes in pig. Pig fetuses (55 and 92 days of gestation) and postnatal piglets (1, 3, 8, 17, 31 and 41 days after birth) were used. RNS production was measured by fluorescent probes diaminofluorescein-diacetate (DAF-FMDA) and dichlorofluorescein-diacetate (H2DCFDA). Levels of nitration of cell proteins were established by immunofluorescent detection of nitrotyrosine. Levels of plasma nitrites/nitrates were detected spectrophotometrically by Griess reaction. Nitric oxide production measured by DAF-FMDA in neutrophils decreased during postnatal life. Spontaneous RNS measured by H2DCFDA decreased from 55th day of gestation to the 41st day of life. Phorbol-12-myristate-13-acetate activated production decreased during postnatal life. Production of NO measured by DAF-FMDA in macrophages decreased from the 1st to 41st day after birth. RNS production measured by H2DCFDA in monocytes did not show
  • The aim of this work was to evaluate ontogeny of reactive nitrogen species (RNS) production by peripheral blood phagocytes in pig. Pig fetuses (55 and 92 days of gestation) and postnatal piglets (1, 3, 8, 17, 31 and 41 days after birth) were used. RNS production was measured by fluorescent probes diaminofluorescein-diacetate (DAF-FMDA) and dichlorofluorescein-diacetate (H2DCFDA). Levels of nitration of cell proteins were established by immunofluorescent detection of nitrotyrosine. Levels of plasma nitrites/nitrates were detected spectrophotometrically by Griess reaction. Nitric oxide production measured by DAF-FMDA in neutrophils decreased during postnatal life. Spontaneous RNS measured by H2DCFDA decreased from 55th day of gestation to the 41st day of life. Phorbol-12-myristate-13-acetate activated production decreased during postnatal life. Production of NO measured by DAF-FMDA in macrophages decreased from the 1st to 41st day after birth. RNS production measured by H2DCFDA in monocytes did not show (en)
Title
  • Ontogeny of reactive nitrogen species production by blood phagocytes in pigs
  • Ontogeny of reactive nitrogen species production by blood phagocytes in pigs (en)
  • Ontogeneze produkce reaktivních forem dusíku krevními fagocyty prasat. (cs)
skos:prefLabel
  • Ontogeny of reactive nitrogen species production by blood phagocytes in pigs
  • Ontogeny of reactive nitrogen species production by blood phagocytes in pigs (en)
  • Ontogeneze produkce reaktivních forem dusíku krevními fagocyty prasat. (cs)
skos:notation
  • RIV/62157124:16170/08:00001840!RIV09-MZE-16170___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA524/05/0267), Z(AV0Z50450515), Z(MSM0021620849), Z(MSM6215712403), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 384882
http://linked.open...ai/riv/idVysledku
  • RIV/62157124:16170/08:00001840
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • NITRIC-OXIDE SYNTHASE; ALVEOLAR MACROPHAGES; URINARY NITRITE; NEWBORN-INFANTS; PRETERM INFANTS; PLASMA NITRITE; L-ARGININE; NITRATE; FETAL; METABOLITES (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [B695C1D54B32]
http://linked.open...i/riv/nazevZdroje
  • Physiological Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 57
http://linked.open...iv/tvurceVysledku
  • Faldyna, Martin
  • Kovářů, H.
  • Ondráček, Jaroslav
  • Kovářů, František
  • Zelníčková, Petra
http://linked.open...ain/vavai/riv/wos
  • 000254999400012
http://linked.open...n/vavai/riv/zamer
issn
  • 0862-8408
number of pages
http://localhost/t...ganizacniJednotka
  • 16170
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