About: The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae

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About: The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Single chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. For scFv selection recombinant Bhlp 29.7 protein was used and individual clones were tested in ELISA and immunofluorescent test. The total of four unique clones was isolated. One of selected clones was able to bind specifically B.hyodysenteriae in ELISA and immunofluorescent test. This is the first report of species specific recombinant antibodies against B. hyodysenteriae. Single chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. For scFv selection recombinant Bhlp 29.7 protein was used and individual clones were tested in ELISA and immunofluorescent test. The total of four unique clones was isolated. One of selected clones was able to bind specifically B.hyodysenteriae in ELISA and immunofluorescent test. This is the first report of species specific recombinant antibodies against B. hyodysenteriae. (en) Rekombinantní scFv protilátky specifické pro druh Brachyspira hyodysenteriae byly izolovány za použití fágové knihovny. Při selekci protilátek proti rekombinantnímu proteinu Bhlp 29,7 byly získány klony, které byly testovány pomocí ELISA testu a imunofluorescenčního testu. Byly získány čtyři unikátní klony. Jeden z těchto klonů byl schopen specificky reagovat s Bhlp 29,7 proteinem B. hyodysenteriae v ELISA testu i IF testu. (cs)
Title	The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae Selekce scFv protilátkových fragmentů specifických k Bhlp 29,7 proteinu Brachyspira hyodysenteriae (cs) The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae (en)
skos:prefLabel	The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae Selekce scFv protilátkových fragmentů specifických k Bhlp 29,7 proteinu Brachyspira hyodysenteriae (cs) The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae (en)
skos:notation	RIV/62157124:16170/08:00001815!RIV09-GA0-16170___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA524/06/1501)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Čížek, Alois Celer, Vladimír Lobová, Dana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinárního lékařství
http://linked.open...dnocenehoVysledku	394173
http://linked.open...ai/riv/idVysledku	RIV/62157124:16170/08:00001815
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Brachyspira hyodysenteriae; recombinant Bhlp 29.7; scFv; ELISA; specificity (en)
http://linked.open.../riv/klicoveSlovo	Brachyspira hyodysenteriae ELISA specificity scFv recombinant Bhlp 29.7
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[2F10CD0529C5]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Recombinant lipoprotein (BmpB) of the outer membrane of B. hyodysenteriae and scFv antibodies as a means of determination of asymptomatic carriers
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Čížek, Alois Celer, Vladimír Lobová, Dana
issn	0015-5632
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	16170

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