About: Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library     Goto   Sponge   NotDistinct   Permalink

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  • Single chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against Lawsonia intracellularis.
  • Single chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against Lawsonia intracellularis. (en)
  • Izolovali a charakterizovali jsme rekombinantní scFv protilátky se specifickou vazbou k Lawsonia intracellularis. K selekci jsme jako antigen využili celé bakteriální buňky L. intracellularis, jednotlivé klony byly testovány ELISA test. Celkem jsme získali 7 klonů těchto protilátek s jedinečnýkm profilem metodou DNA fingerprinting. Všechny klony vykazovaly specifickou vazbu na bakterii v imunofluorescenčním testu. (cs)
Title
  • Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library
  • Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library (en)
  • Izolace scFv protilátek proti Lawsonia intracellularis z fagemidové knihovny (cs)
skos:prefLabel
  • Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library
  • Isolation of Lawsonia intracellularis specific single-chain Fv antibody fragments from phage display library (en)
  • Izolace scFv protilátek proti Lawsonia intracellularis z fagemidové knihovny (cs)
skos:notation
  • RIV/62157124:16170/07:00001430!RIV08-GA0-16170___
http://linked.open.../vavai/riv/strany
  • 85-90
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  • P(GP524/04/P038)
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  • 1
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  • 427648
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  • RIV/62157124:16170/07:00001430
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  • Lawsonia intracellularis; scFv; immunofluorescence; specificity (en)
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  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [CD4FEE595C83]
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  • Research in Veterinary Science
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http://linked.open...v/svazekPeriodika
  • 83
http://linked.open...iv/tvurceVysledku
  • Smola, Jiří
  • Celer, Vladimír
  • Molinková, Dobromila
  • Trundová, Mária
  • Tomanová, Katarína
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  • 0034-5288
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  • 16170
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