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  • Na souboru 400 prasečích sér byla uskutečněna kvantifikace PCV2 viru metodou Real-time PCR a zároveň uskutečněna detekce PCV2 specifických protilátek metodou ELISA. Vzorky pocházely z farem s konvenčním chovem prasat a zároveň s výskytem cirkovirového chřadnutí selat po odstavu. Sledování bylo uskutečněno na selatech pocházejících ze 7 vrhů selat (ve věku 6-25 týdnů), která byla odebírána v intervalu 2-3 týdnů. IgM protilátky byly poprvé zaznamenány ve věku 8 týdnů a dosáhly maximální úrovně ve 12 týdnech stáří; IgG protilátky byly poprvé zjištěny v 10. týdnu a vrcholily v 16. týdnu. (průměrný titr 1:3500). Myximálná titr viru byl zaznamenán ve věku 10 týdnů (7x10(7) kopií genomu/ml séra), virová infekce perzistovala až do dospělého věku. (cs)
  • Porcine circovirus 2 quantification by real-time PCR and determination of virus specific antibodies using a peptide based ELISA test was performed on a total of 400 serum samples. Samples for antibody measurement and virus quantification were obtained from a conventional pig farm with a clinical form of post-weaning multisystemic wasting syndrome. Samples were taken during the post-weaning period. Seven litters of weaned piglets (6-25 weeks) were systematically sampled at 2-3 week intervals. Porcine circovirus 2 specific antibodies were detected using the peptide based ELISA test. IgM antibodies were first detected at week 8 and reached their highest levels at week 12; IgG antibody appeared at week 10 and peaked at week 16 with an average titer at 1:3500. Although, the viral load peaked at week 10 (7x10(7) genomes copy/ml of sera), viral infection persisted through to adult age (10(5) genomes copy/ml of sera).
  • Porcine circovirus 2 quantification by real-time PCR and determination of virus specific antibodies using a peptide based ELISA test was performed on a total of 400 serum samples. Samples for antibody measurement and virus quantification were obtained from a conventional pig farm with a clinical form of post-weaning multisystemic wasting syndrome. Samples were taken during the post-weaning period. Seven litters of weaned piglets (6-25 weeks) were systematically sampled at 2-3 week intervals. Porcine circovirus 2 specific antibodies were detected using the peptide based ELISA test. IgM antibodies were first detected at week 8 and reached their highest levels at week 12; IgG antibody appeared at week 10 and peaked at week 16 with an average titer at 1:3500. Although, the viral load peaked at week 10 (7x10(7) genomes copy/ml of sera), viral infection persisted through to adult age (10(5) genomes copy/ml of sera). (en)
Title
  • The levels of PCV2 specific antibodies and viremia in pigs
  • The levels of PCV2 specific antibodies and viremia in pigs (en)
  • Hladiny PCV2 specifických protilátek a viremie u prasat (cs)
skos:prefLabel
  • The levels of PCV2 specific antibodies and viremia in pigs
  • The levels of PCV2 specific antibodies and viremia in pigs (en)
  • Hladiny PCV2 specifických protilátek a viremie u prasat (cs)
skos:notation
  • RIV/62157124:16170/07:00001419!RIV08-GA0-16170___
http://linked.open.../vavai/riv/strany
  • 274-278
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  • P(GA524/06/1640)
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  • 5
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  • 430926
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  • RIV/62157124:16170/07:00001419
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  • circovirus; antibodies; quantitative PCR; viremia (en)
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  • NL - Nizozemsko
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  • [269CC1534047]
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  • Research in Veterinary Science
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  • 12
http://linked.open...iv/tvurceVysledku
  • Smola, Jiří
  • Celer, Vladimír
  • Lobová, Dana
  • Molinková, Dobromila
  • Trundová, Mária
  • Carasová, Patricie
  • Takáčová, Kristína
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  • 0034-5288
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  • 16170
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