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Description
| - Na souboru 400 prasečích sér byla uskutečněna kvantifikace PCV2 viru metodou Real-time PCR a zároveň uskutečněna detekce PCV2 specifických protilátek metodou ELISA. Vzorky pocházely z farem s konvenčním chovem prasat a zároveň s výskytem cirkovirového chřadnutí selat po odstavu. Sledování bylo uskutečněno na selatech pocházejících ze 7 vrhů selat (ve věku 6-25 týdnů), která byla odebírána v intervalu 2-3 týdnů. IgM protilátky byly poprvé zaznamenány ve věku 8 týdnů a dosáhly maximální úrovně ve 12 týdnech stáří; IgG protilátky byly poprvé zjištěny v 10. týdnu a vrcholily v 16. týdnu. (průměrný titr 1:3500). Myximálná titr viru byl zaznamenán ve věku 10 týdnů (7x10(7) kopií genomu/ml séra), virová infekce perzistovala až do dospělého věku. (cs)
- Porcine circovirus 2 quantification by real-time PCR and determination of virus specific antibodies using a peptide based ELISA test was performed on a total of 400 serum samples. Samples for antibody measurement and virus quantification were obtained from a conventional pig farm with a clinical form of post-weaning multisystemic wasting syndrome. Samples were taken during the post-weaning period. Seven litters of weaned piglets (6-25 weeks) were systematically sampled at 2-3 week intervals. Porcine circovirus 2 specific antibodies were detected using the peptide based ELISA test. IgM antibodies were first detected at week 8 and reached their highest levels at week 12; IgG antibody appeared at week 10 and peaked at week 16 with an average titer at 1:3500. Although, the viral load peaked at week 10 (7x10(7) genomes copy/ml of sera), viral infection persisted through to adult age (10(5) genomes copy/ml of sera).
- Porcine circovirus 2 quantification by real-time PCR and determination of virus specific antibodies using a peptide based ELISA test was performed on a total of 400 serum samples. Samples for antibody measurement and virus quantification were obtained from a conventional pig farm with a clinical form of post-weaning multisystemic wasting syndrome. Samples were taken during the post-weaning period. Seven litters of weaned piglets (6-25 weeks) were systematically sampled at 2-3 week intervals. Porcine circovirus 2 specific antibodies were detected using the peptide based ELISA test. IgM antibodies were first detected at week 8 and reached their highest levels at week 12; IgG antibody appeared at week 10 and peaked at week 16 with an average titer at 1:3500. Although, the viral load peaked at week 10 (7x10(7) genomes copy/ml of sera), viral infection persisted through to adult age (10(5) genomes copy/ml of sera). (en)
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Title
| - The levels of PCV2 specific antibodies and viremia in pigs
- The levels of PCV2 specific antibodies and viremia in pigs (en)
- Hladiny PCV2 specifických protilátek a viremie u prasat (cs)
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skos:prefLabel
| - The levels of PCV2 specific antibodies and viremia in pigs
- The levels of PCV2 specific antibodies and viremia in pigs (en)
- Hladiny PCV2 specifických protilátek a viremie u prasat (cs)
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skos:notation
| - RIV/62157124:16170/07:00001419!RIV08-GA0-16170___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16170/07:00001419
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - circovirus; antibodies; quantitative PCR; viremia (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Research in Veterinary Science
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Smola, Jiří
- Celer, Vladimír
- Lobová, Dana
- Molinková, Dobromila
- Trundová, Mária
- Carasová, Patricie
- Takáčová, Kristína
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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