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| rdf:type
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| Description
| - Cílem práce byla optimalizace metody na stanovení selenu v krvi a tkáních přežvýkavců a praktické zavedení vyvinuté metody do výzkumu a diagnostické praxe. Byla zavedena metoda stanovení selenu hydridovou technikou atomové absorpční spektrofotometrie (HG AAS) v plné krvi, krevní plazmě a séru, játrech, kosterní svalovině, myokardu a ledvinách. Ověření správnosti měření bylo provedeno použitím různých typů referenčních materiálů s deklarovanou koncentrací selenu a metodou měření výtěžnosti přídavku kalibračních roztoků do mineralizovaných vzorků. Výsledky u všech použitých referenčních materiálů odpovídaly deklarovaným hodnotám na 95 % hladině pravděpodobnosti. Uvedená metodika vyhovuje z hlediska detekčního limitu (0,762 mg.l-1) i chyby stanovení (4,6 - 15 %) požadavku pro využití ve výzkumu, klinické i preventivní medicíně hospodářských zvířat. (cs)
- The goal of the study was to optimize the method of blood and tissue selenium determination in ruminants and implement it in research and diagnostic practice. A method for selenium determination by hydride generation atomic absorption spectrophotometry (HG-AAS) in full blood, blood plasma and serum, the liver, skeletal muscle tissue, the myocardium, and the kidneys was developed. The measurements were verified by using diverse types of reference materials with declared selenium concentration and by a method of yield measurement of calibration solution addition in mineralized samples. The results corresponded with the stated values at the 95% probability level for all reference materials used. This methodology is acceptable as to both detection limit (0.762 mg.l-1) and error of measurement (4.6-15%) required for authorized use in research as well as clinical and preventive livestock medicine.
- The goal of the study was to optimize the method of blood and tissue selenium determination in ruminants and implement it in research and diagnostic practice. A method for selenium determination by hydride generation atomic absorption spectrophotometry (HG-AAS) in full blood, blood plasma and serum, the liver, skeletal muscle tissue, the myocardium, and the kidneys was developed. The measurements were verified by using diverse types of reference materials with declared selenium concentration and by a method of yield measurement of calibration solution addition in mineralized samples. The results corresponded with the stated values at the 95% probability level for all reference materials used. This methodology is acceptable as to both detection limit (0.762 mg.l-1) and error of measurement (4.6-15%) required for authorized use in research as well as clinical and preventive livestock medicine. (en)
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| Title
| - Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry
- Stanovení selenu v krvi a tkáních hydridovou technikou atomové absorpční spektrofotometrie (cs)
- Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry (en)
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| skos:prefLabel
| - Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry
- Stanovení selenu v krvi a tkáních hydridovou technikou atomové absorpční spektrofotometrie (cs)
- Blood and tissue selenium determination by hydride generation atomic absorption spectrophotometry (en)
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| skos:notation
| - RIV/62157124:16170/05:00000366!RIV06-MSM-16170___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16170/05:00000366
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - AAS; liver; muscle; myocardium; microwave mineralization (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Pechová, Alena
- Pavlata, Leoš
- Illek, Josef
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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