About: Coprodiagnosis of Hammondia heydorni in dogs by PCR amplification of ITS1 rRNA: Differentiation from morphologically indistinguishable oocysts of Neospora caninum.

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About: Coprodiagnosis of Hammondia heydorni in dogs by PCR amplification of ITS1 rRNA: Differentiation from morphologically indistinguishable oocysts of Neospora caninum. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to Neospora caninum, an important parasite of cattle and dogs. Oocysts of these two species are morphologically indistinguishable from each other. A population of 2240 dogs in the Czech Republic was screened for the presence of H. heydorni / N. caninum oocysts and five (0.22%), represented by five of 3135 faecal samples (0.16%), were positive. The internal transcribed spacer 1 region of the rRNA gene (ITS1) from two isolates were cloned and the DNA sequences were identical with those of the ITS1 of H. heydorni. Based on the rRNA sequences available for H. heydorni and related coccidia, the primer pair JS4-JS5 was designed to amplify the 3' end of thesmall subunit (SSU) rRNA gene and ITS1 of H. heydorni. When tested on DNA extracted from variety of parasites, the primers amplified a specific 267 bp fragment in our isolates only. The presence of DNA equivalent to 10 oocysts was sufficient for the amp Hammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to Neospora caninum, an important parasite of cattle and dogs. Oocysts of these two species are morphologically indistinguishable from each other. A population of 2240 dogs in the Czech Republic was screened for the presence of H. heydorni / N. caninum oocysts and five (0.22%), represented by five of 3135 faecal samples (0.16%), were positive. The internal transcribed spacer 1 region of the rRNA gene (ITS1) from two isolates were cloned and the DNA sequences were identical with those of the ITS1 of H. heydorni. Based on the rRNA sequences available for H. heydorni and related coccidia, the primer pair JS4-JS5 was designed to amplify the 3' end of thesmall subunit (SSU) rRNA gene and ITS1 of H. heydorni. When tested on DNA extracted from variety of parasites, the primers amplified a specific 267 bp fragment in our isolates only. The presence of DNA equivalent to 10 oocysts was sufficient for the amp (en)
Title	Coprodiagnosis of Hammondia heydorni in dogs by PCR amplification of ITS1 rRNA: Differentiation from morphologically indistinguishable oocysts of Neospora caninum. Coprodiagnosis of Hammondia heydorni in dogs by PCR amplification of ITS1 rRNA: Differentiation from morphologically indistinguishable oocysts of Neospora caninum. (en)
skos:prefLabel	Coprodiagnosis of Hammondia heydorni in dogs by PCR amplification of ITS1 rRNA: Differentiation from morphologically indistinguishable oocysts of Neospora caninum. Coprodiagnosis of Hammondia heydorni in dogs by PCR amplification of ITS1 rRNA: Differentiation from morphologically indistinguishable oocysts of Neospora caninum. (en)
skos:notation	RIV/62157124:16170/01:00000731!RIV/2002/MSM/161702/N
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 161700001)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Modrý, David Koudela, Břetislav
http://linked.open.../riv/druhVysledku	M - Uspořádání (zorganizování) konference
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinárního lékařství
http://linked.open...dnocenehoVysledku	676464
http://linked.open...ai/riv/idVysledku	RIV/62157124:16170/01:00000731
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	coprodiagnosis, oocysts, Neospora caninum, PCR (en)
http://linked.open.../riv/klicoveSlovo	oocysts PCR Neospora caninum coprodiagnosis
http://linked.open...ontrolniKodProRIV	[74D50BF042C9]
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http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/statKonaniAkce	US - Spojené státy americké
http://linked.open...iv/tvurceVysledku	Modrý, David Koudela, Břetislav Lukeš, J. Šlapeta, Jan Votýpka, J. Hořejš, R.
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/ukonceniAkce	2001-09-15 (xsd:date)
http://linked.open.../riv/zahajeniAkce	2001-09-13 (xsd:date)
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http://localhost/t...ganizacniJednotka	16170

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