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| Description
| - The aim of this study was to compare two methods of immunoreactive cortisol determination in urine and an assessment of the validity of urine cortisol/creatinine ratio (UCCR) in dogs. The UCCR was measured in three groups of dogs: (1) healthy dogs, (2) dogs with hyperadrenocorticism and (3) dogs suffering from other diseases presented with polyuria and polydipsia (PU/PD). All cortisol measurements in urine were performed using two methods: (1) fluorescence polarisation immunoassay (FPIA) with urine extrction and (2) radioimmunoassay (RIA) without urine extraction. Paired results of 30 cortisol measurements and 30 UCCR were compared using the Wilcoxon test. It was proved that the differences between the two methods (i.e., FPIA with the urine extraction and RIA without urine extraction) were highly statistically significant (p<0.01). While in the cortisol measurement using the FPIA with urine extraction the median of the UCCR amunted to 18.53 x 10-6, in the RIA without urine extraction it was 37.83 x
- The aim of this study was to compare two methods of immunoreactive cortisol determination in urine and an assessment of the validity of urine cortisol/creatinine ratio (UCCR) in dogs. The UCCR was measured in three groups of dogs: (1) healthy dogs, (2) dogs with hyperadrenocorticism and (3) dogs suffering from other diseases presented with polyuria and polydipsia (PU/PD). All cortisol measurements in urine were performed using two methods: (1) fluorescence polarisation immunoassay (FPIA) with urine extrction and (2) radioimmunoassay (RIA) without urine extraction. Paired results of 30 cortisol measurements and 30 UCCR were compared using the Wilcoxon test. It was proved that the differences between the two methods (i.e., FPIA with the urine extraction and RIA without urine extraction) were highly statistically significant (p<0.01). While in the cortisol measurement using the FPIA with urine extraction the median of the UCCR amunted to 18.53 x 10-6, in the RIA without urine extraction it was 37.83 x (en)
- Cílem práce bylo porovnání dvou metod stanovení imunoreaktivního kortizolu a posouzení validity poměru kortizol/kreatinin v moči psů. U tří skupin psů (1) zdravých, (2) s hyperadrenokorticizmem, (3) s jinými onemocněními prezentujícími se polaurií a polydipsií (PU/PD) byl stanoven poměr kortizol/kreatinin (C/C) v moči. Všechna stanovení kortizolu v moči byla prováděna zároveň dvěma metodami: (1) fluorescenční polarizační imunoanalýzou (FPIA) s extrakcí moči, (2) radioimunoanalytickou metodou (RIA) bez extrakce moči. Výsledky 30 párových stanovení kortizolu a 30 párových hodnot poměru C/C byly mezi sebou porovnány. K vyhodnocení byl užit Wilcoxonův test. Ukázalo se, že rozdíly mezi metodami FPIA s extrakcí a RIA bez extrakce jsou statisticky vysoce významné (p < 0,01). Zatímco při stanovení volného kortizolu v moči metodou FPIA s extrakcí moči byl medián poměru C/C 18,53 x 10-6, u metody RIA bez extrakce moči byl 37,83 x 10-6. Dále byly porovnávány hodnoty poměru C/C u jednotlivých skupin mezi sebo (cs)
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| Title
| - Stanovení imunoreaktivního kortizolu v moči psů a jeho validita při diagnostice hyperadrenokorticizmu (cs)
- Immunoreactive cortisol measurement in canine urine and its validity in hyperadrenocorticosm diagnosis
- Immunoreactive cortisol measurement in canine urine and its validity in hyperadrenocorticosm diagnosis (en)
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| skos:prefLabel
| - Stanovení imunoreaktivního kortizolu v moči psů a jeho validita při diagnostice hyperadrenokorticizmu (cs)
- Immunoreactive cortisol measurement in canine urine and its validity in hyperadrenocorticosm diagnosis
- Immunoreactive cortisol measurement in canine urine and its validity in hyperadrenocorticosm diagnosis (en)
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| skos:notation
| - RIV/62157124:16170/00:00000263!RIV06-MSM-16170___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16170/00:00000263
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - hyperadrenocorticism; cortisol; creatinine; RIA; FPIA; urine extraction (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Svoboda, Miroslav
- Kolevská, Jana
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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