About: Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers

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About: Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rapidly developing methods based on molecular markers considerably contribute to the differentiation, characterisation and testing of individuals and their progeny. In our laboratory we mostly use RAPD for the differentiation of the individual clones of long-term-cultivated somatic spruce embryos; lately we have introduced also the AFLP method. The SCAR method is used to eliminate the lower reproducibility of RAPD. For the characterisation of selected clones of somatic spruce embryos, cultivated at our department for a long time, our own clone-specific SCAR markers derived from polymorphous RAPD products were used. The RAPD fragments of DNA were cloned into plasmid vectors, sequenced and specific primers were proposed. Using PCR with these primers we are able to identify effectively the individual clones in our collection. Rapidly developing methods based on molecular markers considerably contribute to the differentiation, characterisation and testing of individuals and their progeny. In our laboratory we mostly use RAPD for the differentiation of the individual clones of long-term-cultivated somatic spruce embryos; lately we have introduced also the AFLP method. The SCAR method is used to eliminate the lower reproducibility of RAPD. For the characterisation of selected clones of somatic spruce embryos, cultivated at our department for a long time, our own clone-specific SCAR markers derived from polymorphous RAPD products were used. The RAPD fragments of DNA were cloned into plasmid vectors, sequenced and specific primers were proposed. Using PCR with these primers we are able to identify effectively the individual clones in our collection. (en)
Title	Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers (en)
skos:prefLabel	Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers Differentiation of individual clones of long-term-cultivated somatic embryos of Norway spruce using DNA markers (en)
skos:notation	RIV/62156489:_____/03:21100009!RIV/2004/GA0/430004/N
http://linked.open.../vavai/riv/strany	412;412
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP521/03/P148), Z(MSM 432100001)
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Havel, Ladislav Hanáček, Pavel Procházka, Stanislav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	603875
http://linked.open...ai/riv/idVysledku	RIV/62156489:_____/03:21100009
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	RAPD; AFLP; SCAR; somatic embryo; Norway spruce (en)
http://linked.open.../riv/klicoveSlovo	SCAR Norway spruce RAPD AFLP somatic embryo
http://linked.open...ontrolniKodProRIV	[ECED28E8CDFA]
http://linked.open...v/mistoKonaniAkce	Barcelona, Spain
http://linked.open...i/riv/mistoVydani	Barcelona, Spain
http://linked.open...i/riv/nazevZdroje	7th International congress of plant molecular biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The study of genetic variability and stability of long term cultures of Norway spruce somatic embryos
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Hanáček, Pavel Havel, Ladislav Procházka, Stanislav
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2003-06-23 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20432100001
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISPMB

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