About: Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers

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About: Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Random amplified polymorphic DNA (RAPD) markers were used for the determination of the genetic stability and relationship of clones of Norway spruce (Picea abies (L.) Karst.) early somatic embryo cultures. RAPD analysis of the genetic relationship was carried out on 16 clones of somatic embryos (14 clones derived in our laboratory and two control clones derived in foreign laboratories). Twenty pre-selected primers produced 88 reproducible polymorphic and monomorphic bands, which were evaluated using the RAPDistance Program 1.04. The genetic stability during in vitro culture was studied on 6 clones derived in our laboratory (Brno, Velká Klajdovka) and two control clones. The genomic DNA was isolated in regular intervals 5 times a year during 4 subsequent years. No genetic instability was determined during the 4-year period using 4 selected primers with the highest polymorphisms. Random amplified polymorphic DNA (RAPD) markers were used for the determination of the genetic stability and relationship of clones of Norway spruce (Picea abies (L.) Karst.) early somatic embryo cultures. RAPD analysis of the genetic relationship was carried out on 16 clones of somatic embryos (14 clones derived in our laboratory and two control clones derived in foreign laboratories). Twenty pre-selected primers produced 88 reproducible polymorphic and monomorphic bands, which were evaluated using the RAPDistance Program 1.04. The genetic stability during in vitro culture was studied on 6 clones derived in our laboratory (Brno, Velká Klajdovka) and two control clones. The genomic DNA was isolated in regular intervals 5 times a year during 4 subsequent years. No genetic instability was determined during the 4-year period using 4 selected primers with the highest polymorphisms. (en)
Title	Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers (en)
skos:prefLabel	Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers Characterisation and determination of genetic stability of early somatic embryo culture clones of Norway spruce using RAPD markers (en)
skos:notation	RIV/62156489:_____/02:21100001!RIV/2003/MSM/430003/N
http://linked.open.../vavai/riv/strany	517;521
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Havel, Ladislav Hanáček, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	640600
http://linked.open...ai/riv/idVysledku	RIV/62156489:_____/02:21100001
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA fingerprinting;genetic stability;Picea abies;RAPD;somatic embryogenesis (en)
http://linked.open.../riv/klicoveSlovo	DNA fingerprinting somatic embryogenesis Picea abies RAPD genetic stability
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[B1788EEA9F54]
http://linked.open...i/riv/nazevZdroje	Biologia
http://linked.open...in/vavai/riv/obor	GC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Hanáček, Pavel Havel, Ladislav Truksa, Martin
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20432100001
issn	0006-3088
number of pages	5 (xsd:int)

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