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  • Successful conversion of mature somatic embryos depends on balanced growth and development of both shoot and root. But the cotyledons and hypocotyl develop first and after that it can be difficult to get the root to grow in normal way. In our experiments we used mature somatic embryos of two Norway spruce cell lines. The root growth of plantlets was stimulated by addition of indolyl-3-butyric acid (IBA) into conversion media. The supplement of 0.1 µM IBA into the first conversion (germination) medium with activated charcoal was the most beneficial and increased root length approximately about 54-86% after 4 weeks. The plantlets were then transferred onto the second conversion medium with lower concentration of IBA, which was 0.05 µM. The roots continued in growth and shoots were also well developed. After two months the plantlets were successfully transplanted into the soil.
  • Successful conversion of mature somatic embryos depends on balanced growth and development of both shoot and root. But the cotyledons and hypocotyl develop first and after that it can be difficult to get the root to grow in normal way. In our experiments we used mature somatic embryos of two Norway spruce cell lines. The root growth of plantlets was stimulated by addition of indolyl-3-butyric acid (IBA) into conversion media. The supplement of 0.1 µM IBA into the first conversion (germination) medium with activated charcoal was the most beneficial and increased root length approximately about 54-86% after 4 weeks. The plantlets were then transferred onto the second conversion medium with lower concentration of IBA, which was 0.05 µM. The roots continued in growth and shoots were also well developed. After two months the plantlets were successfully transplanted into the soil. (en)
Title
  • EFFECT OF IBA ON ROOT GROWTH OF NORWAY SPRUCE SOMATIC EMBRYOS
  • EFFECT OF IBA ON ROOT GROWTH OF NORWAY SPRUCE SOMATIC EMBRYOS (en)
skos:prefLabel
  • EFFECT OF IBA ON ROOT GROWTH OF NORWAY SPRUCE SOMATIC EMBRYOS
  • EFFECT OF IBA ON ROOT GROWTH OF NORWAY SPRUCE SOMATIC EMBRYOS (en)
skos:notation
  • RIV/62156489:_____/01:21100008!RIV/2002/GA0/430002/N
http://linked.open.../vavai/riv/strany
  • 117;117
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GV522/96/K186)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 678685
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:_____/01:21100008
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • IBA; Norway spruce; somatic embryogenesis; in vitro (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [7DFD58FED195]
http://linked.open...v/mistoKonaniAkce
  • Brno, Czech Republic
http://linked.open...i/riv/mistoVydani
  • Brno, Czech Republic
http://linked.open...i/riv/nazevZdroje
  • 17th International Conference on Plant Growth Substances
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Havel, Ladislav
  • Slabý, Karel
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Neuveden
https://schema.org/isbn
  • 80-7157-518-6
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