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  • Embryogenic cell suspension cultures from hypocotyl segments of cucumber (Cucumis sativus L.) have been initiated by 7 days exposure to Murashige and Skoog medium supplemented with 3% sucrose, 2.2 mg l-1 2,4-dichlorophenoxyacetic acid, and 100 mg l-1 casein hydrolyzate. The suspension cultures were maintained in the same medium with 1.1 mg l-1 2,4- dichlorophenoxyacetic acid only on a horizontal shaker at 28°C, and 16/8 h photoperiod, subcultivated every week. The cell suspension cultures contained heterogeneous arrays of several different types of single cells (size < 200 µm) and cell clusters (size ? 200 µm). The average generation time was 3 days. The somatic embryos developed after transfer from auxin-containing medium to auxin-free medium. Embryogenic capacity of the cell lines varied between 35 and 470 somatic embryos per 1 ml of cell suspension.
  • Embryogenic cell suspension cultures from hypocotyl segments of cucumber (Cucumis sativus L.) have been initiated by 7 days exposure to Murashige and Skoog medium supplemented with 3% sucrose, 2.2 mg l-1 2,4-dichlorophenoxyacetic acid, and 100 mg l-1 casein hydrolyzate. The suspension cultures were maintained in the same medium with 1.1 mg l-1 2,4- dichlorophenoxyacetic acid only on a horizontal shaker at 28°C, and 16/8 h photoperiod, subcultivated every week. The cell suspension cultures contained heterogeneous arrays of several different types of single cells (size < 200 µm) and cell clusters (size ? 200 µm). The average generation time was 3 days. The somatic embryos developed after transfer from auxin-containing medium to auxin-free medium. Embryogenic capacity of the cell lines varied between 35 and 470 somatic embryos per 1 ml of cell suspension. (en)
Title
  • INITIATION AND MAINTENANCE OF CUCUMBER (Cucumis sativus L.) EMBRYOGENIC CELL SUSPENSION
  • INITIATION AND MAINTENANCE OF CUCUMBER (Cucumis sativus L.) EMBRYOGENIC CELL SUSPENSION (en)
skos:prefLabel
  • INITIATION AND MAINTENANCE OF CUCUMBER (Cucumis sativus L.) EMBRYOGENIC CELL SUSPENSION
  • INITIATION AND MAINTENANCE OF CUCUMBER (Cucumis sativus L.) EMBRYOGENIC CELL SUSPENSION (en)
skos:notation
  • RIV/62156489:_____/01:21100007!RIV/2002/MSM/430002/N
http://linked.open.../vavai/riv/strany
  • 118;118
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 432100001)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 683039
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:_____/01:21100007
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cucumber; embryogenic cell suspension; in vitro (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [9CC724287353]
http://linked.open...v/mistoKonaniAkce
  • Brno, Czech Republic
http://linked.open...i/riv/mistoVydani
  • Brno, Czech Republic
http://linked.open...i/riv/nazevZdroje
  • 17th International Conference on Plant Growth Substances
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Procházka, Stanislav
  • Andrýsková, Lenka
  • Smíšková, Alexandra
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • Neuveden
https://schema.org/isbn
  • 80-7157-518-6
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