About: Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers     Goto   Sponge   NotDistinct   Permalink

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Description
  • The genetic potential of and relationships among amaranth (Amaranthus spp.) genotypes conserved in gene banks are generally unknown. In this study, nine amplified fragment length polymorphism (AFLP) and 14 inter simple sequence repeats (ISSR) primers were used to study genetic diversity among amaranth species. A total of 92 ISSR loci, of which 86 were polymorphic, were detected. The bands ranged from 300 bp to 2000 bp with polymorphic information content (PIC) of 047. The AFLP primer combinations revealed a total of 648 bands, with 489 as the number of polymorphic loci. The fragments ranged from 52 bp to 423 bp in length with PIC of 0.48. The correlation between genetic similarity based on ISSR and AFLP was 0.77: thus, 59% of the relationship was explained. The cluster analysis revealed distinct groups of amaranth genotypes. Therefore, the use of molecular markers would be valuable for effective utilization of amaranth in breeding programs.
  • The genetic potential of and relationships among amaranth (Amaranthus spp.) genotypes conserved in gene banks are generally unknown. In this study, nine amplified fragment length polymorphism (AFLP) and 14 inter simple sequence repeats (ISSR) primers were used to study genetic diversity among amaranth species. A total of 92 ISSR loci, of which 86 were polymorphic, were detected. The bands ranged from 300 bp to 2000 bp with polymorphic information content (PIC) of 047. The AFLP primer combinations revealed a total of 648 bands, with 489 as the number of polymorphic loci. The fragments ranged from 52 bp to 423 bp in length with PIC of 0.48. The correlation between genetic similarity based on ISSR and AFLP was 0.77: thus, 59% of the relationship was explained. The cluster analysis revealed distinct groups of amaranth genotypes. Therefore, the use of molecular markers would be valuable for effective utilization of amaranth in breeding programs. (en)
Title
  • Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers
  • Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers (en)
skos:prefLabel
  • Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers
  • Genetic Diversity Assessment in Amaranth Germplasm using AFLP and ISSR Markers (en)
skos:notation
  • RIV/62156489:43510/14:00231398!RIV15-MSM-43510___
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  • 18093
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  • RIV/62156489:43510/14:00231398
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  • DNA polymorphism; Amaranthus spp.; molecular marker; genetic similarity (en)
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  • US - Spojené státy americké
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  • [BEA57FEA6033]
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  • Journal of Crop Improvement
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  • 28
http://linked.open...iv/tvurceVysledku
  • Baránek, Miroslav
  • Čechová, Jana
  • Isaac, Daniel
  • Oduwaye, Oluwasegun
  • Ojo, David
  • Popoola, Akinola
issn
  • 1542-7528
number of pages
http://bibframe.org/vocab/doi
  • 10.1080/15427528.2014.921262
http://localhost/t...ganizacniJednotka
  • 43510
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